耐碳青霉烯类肠杆菌科细菌KPC-2与NDM-1基因的研究  

Investigation of blaKPC-2 and blaNDM-1 of carbapenem-resistant Enterobacteriaceae

作　　者：谢小芳[1] 沈海英[2] 周惠琴[1] 杨欢[1] 朱雪明[1] 杜鸿[1] 

机构地区：[1]苏州大学附属第二医院检验科,江苏苏州215004 [2]苏州市药品检验所,江苏苏州215104

出　　处：《中华医院感染学杂志》2014年第11期2601-2603,2606,共4页Chinese Journal of Nosocomiology

基　　金：苏州大学附属第二医院青年预研基金项目(SDFEYQN1106)

摘　　要：目的检测耐碳青霉烯类抗菌药物肠杆菌科细菌产酶情况以及KPC-2和NDM-1耐药基因的检出,分析耐碳青霉烯类抗菌药物的耐药机制。方法采用改良Hodge试验、亚胺培南-EDTA纸片协同试验和AmpC酶三维试验检测29株耐碳青霉烯类抗菌药物肠杆菌科细菌产酶情况;PCR法检测KPC-2及NDM-1两种碳青霉烯酶耐药基因。结果 29株分离菌中26株菌改良Hodge试验阳性,7株亚胺培南-EDTA纸片协同试验阳性,7株AmpC酶三维试验阳性,16株携带KPC-2型碳青霉烯酶耐药基因,占55.17%,未扩增出NDM-1碳青霉烯酶耐药基因。结论耐碳青霉烯类抗菌药物肠杆菌科细菌的耐药机制主要是产碳青霉烯酶。OBJECTIVE To detect the situation of enzyme production in carbapenem-resistance Enterobacteriaceae and the detected blaKPC and blaNDM-1, and to investigate the mechanism of carbapenem-resistance in Enterobacteriaceae. METHODS The situation of enzyme production in 29 strains of carbapenem-resistance Enterobacteriaceae was detected by modified Hodge test and imipenem-EDTA double- disk synergy test and three- dimensional modified test. blaKPC and blaNDM-1, these two carbapenemases-resistance genes were analyzed by PCR and verified by DNA sequencing. RESULTS A total of 26 strains from 29 isolated strains were positive in modified Hodge test, 7 strains positive in imipenem-EDTA double- disk synergy test, 7 strains positive in three- dimensional modified test. There were 16 strains carrying blaKPC carbapenemase-resistant genes, accounting for 55.17% and no strains carrying blaNDM-1 carbapenemase-resistant gene. CONCLUSIONS The mechanism of drug resistance of earbapenem-resistant Enterobacteriaceae is mainly due to production of earbapenemase.

关 键 词：肠杆菌科细菌 碳青霉烯酶 KPC-2基因 NDM-1基因 

分 类 号：R378.2[医药卫生—病原生物学]