七氟醚吸入麻醉对老年大鼠认知影响  

Effects of anesthetic sevoflurane on cognitive function in aged rats

作　　者：姜梅[1] 宫建[2] 于嵩[3] 杨朋[4] 邱镜石 吴秀英[5] 

机构地区：[1]沈阳医学院沈洲医院,辽宁沈阳110002 [2]沈阳药科大学生科院临床药学教研室 [3]辽宁中医药大学解剖组胚教研室 [4]沈阳市第四人民医院干诊科 [5]中国医科大学盛京医院麻醉科

出　　处：《中国公共卫生》2014年第6期745-747,共3页Chinese Journal of Public Health

基　　金：沈阳医学院科学研究基金(20131016)

摘　　要：目的观察七氟醚吸入麻醉对大鼠的认知与海马乙酰胆碱影响。方法 20月龄雄性Sprague-Dawley大鼠60只随机分为3组,每组20只:对照组,七氟醚吸入低剂量组(1%),七氟醚吸入高剂量组(2%),每组20只,七氟醚连续吸入5 d后进行Morris水迷宫测试,于测试后第1、4 d和第7 d测定大鼠认知功能及海马乙酰胆碱含量。结果 3组大鼠潜伏期和总路程随测试时间延长呈下降趋势,但七氟醚吸入大鼠较正常大鼠认知能力增强得较慢七氟醚吸入第4、7 d,低剂量组、高剂量组、潜伏期、总路程,均明显低于对照组(F=11.10、17.47;F=49.23、202.74,P均<0.01),且海马乙酰胆碱含量均明显低于对照组(F=30.67、24.38,P均<0.01)。结论七氟醚可能通过改变老年大鼠海马乙酰胆碱的含量损伤其认知功能。Objective To investigate the effects of sevoflurane anesthesia on cognitive function and the expression of acetylcholine in aged rats. Methods Sixty Sprague-Dawley rats were randomly divided into 3 groups：control group, 1% sevoflurane group, and 2% sevoflurane group. All rats received anesthesia for 5 days. Cognitive function was tested with Morris water maze. Acetylcholine expression in the hippocampus was determined with enzyme-linked immunosorbent assay（ELISA）. Results Morris water maze test demonstrated that both latency and the total distance for hidden-platform were significantly decreased in all groups, while the cognitive function in the sevoflurane groups was lower than that of the controls. The latency and total distance for the rats in 1% and 2% sevoflurance groups were significantly lower than those of the rats in the control group at day 4 and day 7 of the treatment （ at day 4 ： F = 11.10, F = 17.47 ; at day 7 ： F = 49.23, F = 202.74;P 〈 0.01 for all）. The expression of acetylcholine in the hippocampus of the sevoflurane groups was significantly lower than that of control group （ F = 30. 67 at day 4, F = 24. 38 at day 7 ; P 〈 0. 01 for all）. Conclusion Sevoflurane could impair cognitive function of aged rats through inhibiting the expression of acetylcholine in the hippocampus.

关 键 词：七氟醚 Moms水迷宫 术后认知功能障碍 乙酰胆碱 

分 类 号：R996[医药卫生—毒理学]