离心力竭运动对大鼠骨骼肌肌浆网Ca^(2+)-ATP酶活性的影响  

Effect of acute eccentric exercise on skeletal muscles sarcoplasmic reticulumin Ca^(2+)-ATPase activity in rats

作　　者：刘建军 孙岳 

机构地区：[1]辽宁省体育科学研究所,辽宁沈阳110179

出　　处：《辽宁体育科技》2013年第6期22-25,共4页Liaoning Sport Science and Technology

摘　　要：目的:采用大鼠下坡跑运动损伤模型,研究离心力竭运动后不同时刻大鼠骨骼肌肌浆网Ca2+-ATP酶活性的变化,探讨离心力竭运动所致骨骼肌超微结构损伤机制,为科学运动训练及运动恢复提供实验和理论依据。方法:雄性SD大鼠60只随机分为6组(每组10只):安静对照组、运动后即刻组、12h组、24h组、48h组和72h组,以速度16m/min,坡度-16°进行跑台运动,运动100min,休息5min,再运动100min,在不同时刻观察大鼠肱三头肌肌浆网Ca2+-ATP酶活性的变化。结果:运动后即刻肌浆网Ca2+-ATP酶活性与对照组相比显著下降(P<0.01),随后开始恢复,运动后24h接近对照组水平(P>0.05),运动后48h已完全恢复到对照组水平。结论:离心力竭运动后即刻大鼠肱三头肌肌浆网Ca2+-ATP酶活性显著下降,随后逐渐恢复,运动后24h明显恢复,至48h已完全恢复,肌浆网Ca2+-ATP酶活性的变化可以间接评定运动后骨骼肌的损伤。Purpose： Exercise induced muscle ultrastructural injury from acute and unaccustomed eccentric exercise. This study used rats classic downhill run sports injury model, different time after injury in rat skeletal muscle sarcoplasmic reticulum Ca^2＋-ATPase activity changes were studied, to explore the mechanism of skeletal muscle injury from acute eccentric exercise, and provide a theoretical basis for scientific training. Methods：Sixty adult male Sprague-Dawley rats are divided into six groups at random： control group（n=10）;treadmill exercise group（n=50）,which was further divided into 0h、12h、24h、48h、72h groups. Exercise group performed 200 min treadmill exercise at 16m/min, on a-16°incline, with a 5 min rest period between two 100 min running bouts. The muscle samples were taken immediately, 12h, 24h, 48h and 72h after exercise, then investigate rats＇ triceps skeletal muscle sarcoplasmic reticulum Ca^2 ＋-ATPase activity changes in different time. Results： The sarcoplasmic reticulum Ca^2＋-ATP activity immediately after exercise decreased significantly compared with the control group（P〈0.01）, and then restores gradually, 24 hours after exerciserestored approach to the control group（P 〈0.05）, at 48 hours after exercise has been fully restored to control group level. Conclusions： After acute eccentric exercise rat skeletal muscle sarcoplasmic reticulum Ca^2＋-ATPase activity can be a indirect indicators of rat＇s skeletal muscle injury after eccentric exercise.

关 键 词：大鼠 离心运动 骨骼肌损伤 肌浆网CA^2+-ATP酶 

分 类 号：G804.7[文化科学—运动人体科学]