人参皂甙对红系、粒单系、巨核系细胞株的增殖及转录因子的诱导作用  

Effect of Ginsenosides in Inducing Proliferation and Transcription Factor of Erythrocytic, Granulo-Monocytic and Megakarocytic Cell Lines

作　　者：陈小红[1] 高瑞兰[1] 徐卫红[1] 金锦梅[1] 林筱洁[1] 

机构地区：[1]浙江省中医院血液病研究室,杭州310006

出　　处：《中国中西医结合杂志》2001年第1期40-42,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局课题! (No .972 0 2 5);浙江省自然科学基金会!(No .398499)

摘　　要：目的 :通过观察人参皂甙 (GS)对c fos、GATA 1转录因子的诱导作用 ,探讨GS在造血细胞内的作用机理。方法 :选用粒系细胞株HL 60、单核细胞株U937、红系细胞株K562和巨核系细胞株Meg 0 1作靶细胞 ,MTT法和祖细胞集落培养法观察GS的增殖效应 ;用WesternBlot来分析核蛋白抗原与c fos、GATA 1抗体的结合反应。结果 :( 1)MTT法和祖细胞集落培养法均显示GS( 10 μg/ml)能够明显地刺激三系细胞增殖 ,与对照组比较差异有显著性 (P <0 0 5)。 ( 2 )WesternBlot分析表明HL 60、K562和Meg 0 1细胞经GS处理后核内c fos转录调控蛋白量分别是未经处理细胞的 1 5、2 0和 2 5倍 ,但U937细胞不表达c fos蛋白。 ( 3)除了U937细胞不表达GATA 1蛋白外 ,其他细胞株经GS刺激后GATA 1转录调控蛋白量分别是处理前的 1 5、2 1和 1 3倍。结论 :GS能够明显地刺激 3种系列的细胞株增殖 ,并能有效地通过诱导c fos或GATA 1转录因子而调控造血细胞与增殖或分化有关基因的表达。To investigate the action of Ginsenosides (GS) in inducing transcription factor c-fos and GATA-1 to explore the mechanism of GS in hematopoietic cells. Methods: The proliferation effects of GS on granulocytic (HL-60), monocytic (U937), erythrocytic (K562) and megaryocytic (Meg-01) cell lines were observed by using proliferation test of MTT and colony formation of progenitor cells. The combining reaction of transcription factors c-fos and GATA-1 with nuclear protein antigen were analyzed by Western Blot after being treated by GS. Results: (1) GS (10 μg/ml) could stimulate and promote proliferation of 3 cell lines with significant difference between GS and non-GS control (P<0 05 in all) in both MTT test and colony assay. (2) After treatment with GS, c-fos protein in HL-60, K562 and Meg-01 cell lines was increased by 1.5, 2.0 and 2.5 fold respectively, while U937 cell did not express c-fos. (3) Except that U937 cell hadn′t expressed GATA-1, the other cell lines after the treatment by GS, the GATA-1 protein level was elevated to 1.5, 2.1 and 1.3 fold of that before treatment. Conclusion: The proliferation of three lines initiated by GS was involved in transcription factor c-fos or GATA-1, which could pay the role in the GS induced up-regulation correlated with proliferation and differentiation of hematopoiesis.

关 键 词：人参皂甙 c-fos转录因子 GATA-1转录因子 

分 类 号：R285.5[医药卫生—中药学]