抗人心肌线粒体抗体在病毒性心肌炎和扩张型心肌病中的检测及其意义  

Detection and Significance of Antibody Against Human Myocardial Mitochondria in Virus Myocarditis and Dilated Cardiomyopathy

作　　者：丁建东[1] 吴翔[2] 顾勇[2] 

机构地区：[1]南京医科大学,攻读博士研究生210029 [2]南通医学院附属医院心内科,江苏省224700

出　　处：《中国循环杂志》2001年第1期38-40,共3页Chinese Circulation Journal

基　　金：江苏省科委社会学术基金!资助 (BS970 2 9)

摘　　要：目的 :检测病毒性心肌炎 (VMC)和扩张型心肌病 (DCM)患者血清中是否有抗人心肌线粒体抗体存在及其意义。　　方法 :以人心肌线粒体作抗原 ,应用免疫点印迹在 2 9例 VMC(VMC组 )、2 4例 DCM(DCM组 )、33例其它心脏病(OCD组 )及 2 0例健康献血者 (HBD组 )进行抗人心肌线粒体抗体检测 ,用免疫印迹方法检测其抗原分子量。　　结果 :VMC组 (12例 ,41.4% ) DCM组 (10例 ,41.7% )抗人心肌线粒体抗体阳性明显高于 OCD组 (2例 ,6 .1% )及HBD(0 % ) ;2抗体阳性的 VMC和 DCM患者中有 43.8%的心肌肌钙蛋白 T升高 ,抗体阴性者为 12 .0 % (P<0 .0 5 )有显著性差异 ;3人心肌线粒体特异性抗原的分子量为 30 KD。　　结论 :1在 VMC和 DCM患者血清中存在抗人心肌线粒体抗体 ,说明部分 VMC及 DCM与自身免疫有关 ,该抗体是引起心肌损伤的因素之一 ,对该抗体的检测可作为 VMC和 DCM的辅助诊断手段之一 ;2该抗体的特异性抗原可能为人心肌线粒体腺苷酸转位酶。Objectives:The present study in vestigated the presence and significance of serum antibody against human myocardial mitochondria in patients with virus myocarditis (VMC) and dilated cardiomyopathy (DCM). Methods:The human myocardial mitochondtia were used as antigen to detect serum autoantibody by immuno dot blot in 29 VMC patients,24 DCM patients,33 patients with other cardiac (OCD) and 20 healthy blood donor (HBD).The antigen molecular weight was detected by Western blot. Results:①Positive autoantibody against human myocardial mitochordria was found in 41.4% in VMC and 41.7% in DCM patients,which were much higher than 6.1% in OCD or 0% in HBD patients.②Cardiac troponin T was elevated in 43.8% of autoantibody positive VMC and DCM patients,which was much higher than 12.0% in autoantibody negative VMC and DCM patients ( p <0.05).③The antigen molecular weight of human myocardial mitochondria was 30KD. Conclusions:①The presence of autoantibody against supports that concept that is responsible for the development of VMC and DCM.Autoantibody is one of the factors that give rise to cardiac injury.The antibody detection may serve as a diagnostic index for VMC and DCM.②The specific antigen is probably human myocardial adenine nucleotide translocator.

关 键 词：病毒性心肌炎 扩张型心肌病 抗线粒体抗体 检测 

分 类 号：R542.21[医药卫生—心血管疾病]