诃子多糖提取工艺的研究  

STUDY ON EXTRACT METHOD OF POLYSACCHARIDE FROM TERMINALIA CHEBULA RETZ.

作　　者：牛梦莉[1] 王静[1] 王晋[1] 

机构地区：[1]内蒙古医科大学附属人民医院药剂科,内蒙古呼和浩特010020

出　　处：《内蒙古医科大学学报》2014年第2期148-151,共4页Journal of Inner Mongolia Medical University

基　　金：内蒙古自治区科技计划(社发)项目(2011)

摘　　要：目的:诃子为使君子科诃子及绒毛诃子的干燥成熟果实。诃子所含的活性成分种类很多,研究表明其主要包括鞣质类、三萜皂苷类、黄酮类、糖类等成分。多糖作为诃子中一类活性成分,已有研究表明其具有免疫调节、抗氧化等作用,因此诃子多糖的提取的工艺研究具有深远的理论意义与应用价值。方法:本实验使用了冷浸法提取多糖、紫外分光光度法测定多糖的含量。并通过单因素实验,以多糖提取率作为指标,选取加水倍数A,冷浸时间B、冷浸次数C三个因素设计正交试验。结果:实验结果表明,影响多糖提取率的主次因素为A>C>B,最佳提取工艺为加水倍数3倍、冷浸8 h、冷浸一次。结论:本实验优选出的最佳提取工艺条件,为开发诃子多糖提供了科学依据。Objective：Terminalia chebula Retz. is dry ripened fruit of Terminalia chebula Retz. and Terminalia chebula Retz. var. tomentella kurt. Various active constituents are contained in Terminalia chebula Retz. such as tannins, triterpenoid saponins, flavonoids and saccharides etc. approved by researches. Polysaccharide is a kind of active constituent of Terminalia chebula Retz. with effect of im-munoregulation and antioxidation. Polysaccharide extract method from Terminalia chebula Retz. is with theoretical meaning and application value. Methods： Maceration extract and ultraviolet spectrophotometry are applied in experiments of this paper to determine polysaccharide content. In addition,single factor experiment is applied in this paper with extraction rate of polysaccharide as target,orthogonal test is designed with water amount as A, maceration time as B and maceration frequency as C. Results：The experiments show that major factor influenced extraction rate of polysaccharide is A,followed with C and B as smallest influential factor. Best extract method is with three times water amount in one time of eight hours maceration. Conclusion：Best extract method is selected with experiments of this paper with scientific basis provided for development of Terminalia chebula Retz.

关 键 词：诃子 多糖 冷浸法提取 提取工艺 TERMINALIA chebula retz 

分 类 号：R943.1[医药卫生—药剂学]