有机磷酸酯类化合物急性中毒对大鼠GABA受体的影响  

Effect of organophosphates on γ-aminobutryic acid receptor in acut e poisoning rats

作　　者：彭俊华[1] 张峰[1] 齐心亮[1] 曹波 高宁 

机构地区：[1]兰州军区兰州总医院检验科,730050 [2]第三军区大学预防医学系环境卫生教研室

出　　处：《兰州医学院学报》2001年第1期11-13,共3页Journal of Lanzhou Medical College

摘　　要：目的　探讨有机磷酸酯类化合物急性中毒的γ 氨基丁酸能系统机理。方法　采用大鼠脑GA BA受体为模型 ,从体外、体内实验 (动物抽搐发作和不发作 ) ,观察GABA受体的变化。结果　在体外实验中 ,10mmol·L- 1的梭曼、丙氟磷或二嗪农均能显著降低大鼠GABA受体的结合 (P <0 0 1) ,1mmol·L- 1的梭曼或二嗪农仍能显著降低GABA受体的结合 (P <0 0 5)。大鼠皮下注射梭曼 99μg·kg- 1,或二嗪农灌胃 1 0 95g·kg- 1,发现3H GABA的结合值在抽搐发作时显著降低 ;大鼠腹腔注射丙氟磷 6mg·kg- 1,3H GABA的结合值显著降低。结论　有机磷酸酯类化合物在一定浓度下能显著降低大鼠脑GABA受体的结合。Objective To study th e mechanism of GABA receptor of organophosphates'acute poisoning.Methods The model of rats microsac was used t o observe the change of 3H-GABA binding to GABA receptor with organopho sphates acute poisoning in vitro and in vivo.Results The 3H-GABA total binding of rat s microsac was significant decreased when soman and diisopropylfluorophosphate(D FP) or diazinon were acted on the rat's microsac at 10 mmol·L -1 in vitro ( P <0 01).Soman or diazinon at 1 mmol·L -1 ,the 3H-GABA to tal binding were still significant decreased( P <0 05).Soman (99 μg·kg -1 ,sc.)or diazinon(1 095 g·kg -1 ,ig.),the 3H-GABA to tal binding was significant decreased ( P <0 05) when rat's symptoms happen ing.DFP(6 mg·kg -1 ,i p.),the 3H-GABA total binding was significa n t decreased ( P <0 01).Couclusion The tota l binding of GABA receptor was decreased when organophosphates were acted on the rat's microsac.

关 键 词：有机磷酸酯类化合物 Γ-氨基丁酸受体 急性中毒 预防 治疗 

分 类 号：R595.4[医药卫生—内科学]