罗布麻总RNA提取方法比较研究  

Comparative Research of Total RNA Extraction Methods FromApocynum venetumL.

作　　者：李苗[1,2] 李国旗[1] 

机构地区：[1]宁夏大学西北退化生态系统恢复与重建教育部重点实验室,宁夏银川750021 [2]宁夏农业生物技术重点实验室,宁夏银川750002

出　　处：《北方园艺》2014年第11期93-96,共4页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2011BAC07B03)

摘　　要：以Trizol法、改进SDS法及RNA plant plus Reagent试剂盒法提取罗布麻的总RNA,并通过紫外分光光度法和凝胶电泳检测对其提取效果进行比较分析,以筛选出罗布麻总RNA最佳提取方法。结果表明:Trizol法无法有效获得罗布麻总RNA,有较为显著的降解现象;改进SDS法提取的罗布麻RNA总量较少、浓度低,且试验重复性差;RNA plant plus Reagent试剂盒法提取的RNA质量好,纯度高,完整性强,可直接作为后续相关分子生物学试验材料。Total RNA was extracted by Trizol, modified SDS and RNA plant plus Reagent Kit methods, the extracted effect were compared by UV Spectrophotometry and Gel Electrophoresis, in order to screen out the best method for extracting Apocynum venetum L.. The results showed that the RNA samples extracted by the method of Trizol were degraded gravely. Samples extracted by the method of modified SDS were low concentration and less amount, and the operation results were in poor repeatability. RNA plant plus Reagent Kit method was optimum extraction of A. veneturn L. total RNA. The RNA samples extracted by the method of RNA plant plus Reagent Kit method were high quality, purity and good reproducibility ,and could be used for research of molecule biology directly.

关 键 词：罗布麻 TRIZOL法 改进SDS法 RNA PLANT PLUS Reagent试剂盒法 总RNA提取 

分 类 号：S563.7[农业科学—作物学]