彝药猪鬃草中总黄酮提取工艺研究  

Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris

作　　者：杨申明[1,2] 王振吉[1,2] 沈继森 

机构地区：[1]楚雄师范学院化学与生命科学学院,云南楚雄675000 [2]云南省高校应用生物学重点实验室,云南楚雄675000

出　　处：《北方园艺》2014年第11期125-129,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31300370);云南省应用基础计划资助项目(2011FZ186);云南省应用基础计划项目青年资助项目(2012FD049);云南省重点建设学科;楚雄师范学院重点建设学科基金资助项目(05YJJSXK03);云南省高校科技创新团队支持计划资助项目(IRTSTYN);楚雄师范学院基金资助项目(10YJYB02)

摘　　要：以猪鬃草为试材,采用Al(NO3)3-NaNO2比色法测定了猪鬃草总黄酮的含量,研究不同提取方法对总黄酮提取率的影响,并通过单因素试验和正交实验,以猪鬃草总黄酮提取率为指示,探讨了乙醇体积分数、料液比、提取功率、提取时间对提取率的影响,以优化猪鬃草总黄酮的提取工艺。结果表明:采用微波法提取猪鬃草中总黄酮提取率大、操作简单、准确度较高、回收率好,可作猪鬃草总黄酮含量提取方法;最佳提取工艺参数为乙醇体积分数60%、料液比1∶40g/mL、提取功率1 000W、提取时间30s;在该条件下测得猪鬃草中总黄酮提取率为4.817%,平均加样回收率为99.48%,RSD=0.23‰(n=5)。Taking Adianturn capillus-veneris as material,total flavonoid content was measured by using AI（NO3 ）3-NaNO2 colorimetrie method,effect of different methods on extraction rate of total flavonoid was studied. Based on the single factor experiment, using L9 （3^4 ）orthogonal experiment,with the total flavonoids extraction rate as the indicator, effect of ethanol concentration, solid-liquid ratio, extraction power, extraction time on the extraction rate was studied in order to optimize the extraction technology of total flavonoids from Adianturn capillus-veneris. The results showed that the extraction rate of total flavonoids was the highest by using microwave method and it was simple operation,high accuracy and reliable. The optimum extraction process parameters were as followed：the volume fraction of ethanol 60% ,ratio of material to liquid 1 ： 40 g/mL, extraction power 1 000 W, and extraction time 30 s. The measured total flavonoids extraction rate was 4. 817% under such condition,and the average recovery was 99. 48% ,RSD=2. 30%0（n=5）.

关 键 词：猪鬃草 总黄酮 微波法提取 正交实验 

分 类 号：S682.35[农业科学—观赏园艺]