木蝴蝶药材的质量控制方法研究  

Methods for Quality Control of Oroxylum indicum

作　　者：温献业[1] 刘光明 林善远 

机构地区：[1]广东省医学科学院广东省人民医院,广州510080 [2]广东省新兴中药学校,广东新兴527400

出　　处：《中国药房》2014年第23期2145-2147,共3页China Pharmacy

基　　金：广东省科技计划项目(No.2009B060600009)

摘　　要：目的：建立木蝴蝶药材的质量控制方法。方法：按照2010年版《中国药典》（一部）方法测定不同产地木蝴蝶药材中的灰分、酸不溶性灰分与杂质的含量；采用高效液相色谱法测定药材中木蝴蝶苷B的含量：色谱柱为Promosil C18（200mm×4．6mm，5μm），流动相为甲醇-水-磷酸（42：58：0．2，V／V／V），流速为1．0ml／min，柱温为25℃，检测波长为254nm。结果：4个不同产地木蝴蝶药材的总灰分百分含量范围为2．16％～3．99％；酸不溶性灰分范围为0．01％～0．86％；杂质范围为0．26％～0．52％；木蝴蝶苷B质量分数范围为4．49％～6．14％。结论：所建立方法可用于木蝴蝶药材的质量控制。OBJECTIVE： To establish the methods for quality control of Oroxylum indicum. METHODS ： The contents of ash, acid-insoluble ash and impurities in O. indicum from different producing areas were determined according to Chinese Pharmacopoeia （2010 edition）. The content of oroxin B was detected by HPLC. The determination was performed on Promosil C18 （200 mm× 4.6 mm, 5 μm） column with mobile phase consisted of methanol-water-phosphoric acid （42 ： 58 ： 0.2, V/V/V） at the flow rate of 1.0 ml/ min. The column temperature was 25 ℃, and the detection wavelength was set at 254 nm. RESULTS ： The contents of total ash in 0. indicum from 4 producing areas were between 2.16% and 3.99%; the contents of acid-insoluble ash were between 0.01% and 0.86%; the contents of impurities were between 0.26% and 0.52%; the contents of oroxin B were between 4.49% and 6.14%. CONCLUSIONS ： The method can be used for the quality control of O. indicum.

关 键 词：木蝴蝶 质量控制 木蝴蝶苷B 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]