两面针提取物抗肝癌谱-效关系研究  

Antihepatoma spectrum-effect relationship of extract from Zanthoxyli Radix

作　　者：王宏虹[1,2] 刘华钢[2] 黄慧学[2] 刘丽敏[2] 申庆荣[3] 曹利娟[4] 

机构地区：[1]柳州市妇幼保健院,广西柳州545001 [2]广西医科大学药学院,广西南宁530021 [3]广西壮族自治区人民医院,广西南宁530021 [4]柳州市人民医院,广西柳州545001

出　　处：《中草药》2014年第8期1102-1109,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金地区科学基金项目(30760302)

摘　　要：目的研究两面针药材提取物HPLC指纹图谱与抗肝癌活性之间的关系。方法以人肝癌细胞株HepG2为药效模型,采用MTT法测定33批不同产地两面针药材提取物的抗肝癌活性,采用逐步后退法将量化特征峰面积与MTT法测得两面针提取物抗肿瘤活性的结果结合,构建两面针药材提取物抗肝癌的谱效关系方程。结果建立的两面针药材提取物HPLC指纹图谱相似度达到0.7以上,经相似度软件筛选出24个特征指纹峰,用逐步后退法拟合两面针药材提取物抗肝癌谱效关系方程,该方程具有统计学意义和实际意义,可较准确地预测两面针药材提取物的抗肝癌活性。结论两面针药材提取物抗肝癌活性是多种成分协同、互补、制约相互作用的结果,根据方程可预测肿瘤细胞的敏感性。Objective To study the relationship between HPLC fingerprint and antihepatoma activity of the extract from Zanthoxyli Radix. Methods Zanthoxyli Radix fingerprint was established and human hepatic carcinoma cell line HepG2 was chosen as efficacy model. The antihepatoma activity of the extracts in Zanthoxyli Radix of 33 batchs from different habitats was determined by MTr method. ARer integrated the area of characteristic peak with the antihepatoma activity of Zanthoxyli Radix, the hepatic carcinoma ＂spectrum-effect＂ relationship was established by step-back technique. Results TheHPLC-fingerprint ofZanthoxyli Radix was established,andthe similarity of fmgerprinthad reached above 0.7. All data were done with the chromatographic fingerprint similarity evaluation system software, chosen out 24 fingerprint peaks. The spectrum-effect relationship on HepG2 was established and this relationship was significantly different. The results indicated that the multiple linear regression could predict the antihepatoma activity to HepG2 with statistical and practical significance. Conclusion The spectrum-effect relationship on HepG2 has the good predictive and describing abilities to evaluate the quality and therapeutic activity of Zanthoxyli Radix. According to the spectrum-effect relationship equation, we can predict the sensibility of different tumor cells.

关 键 词：两面针药材 HEPG2细胞 谱效关系 抗肝癌 指纹图谱 

分 类 号：R285.5[医药卫生—中药学]