HPLC-ELSD法测定红景天中红景天苷及大花红天素  

Determination of salidroside and crenulatin in Rhodiolae Sacrae Herbra by HPLC-ELSD

作　　者：伍小艳[1,2] 张桥[3,2] 杨素德[3,2] 孙永成[3,2] 张广仁[1,2] 王振中[3,2] 毕宇安[3,2] 萧伟[3,2] 

机构地区：[1]南京中医药大学,江苏南京210000 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中草药》2014年第8期1157-1159,共3页Chinese Traditional and Herbal Drugs

基　　金：国家科技部重大新药创制(2011ZX09401-097)

摘　　要：目的建立同时测定红景天药材中红景天苷、大花红天素的方法,比较了不同批次药材中这2种成分的质量分数差异。方法采用HPLC-ELSD法,Kromasil C18（250 mm×4.6 mm,5μm）为色谱柱,以乙腈-水为流动相,梯度洗脱,体积流量为1.0 mL/min,柱温为30℃;氮气体积流量2.0 L/min,漂移管温度45℃,增益为2。结果红景天苷进样量在0.806~8.06μg呈良好线性关系（r=0.999 6,n=6）,平均回收率为100.56%;大花红天素进样量在2.142~21.42μg呈良好线性关系（r=0.999 9,n=6）,平均回收率为100.40%。结论该方法简便准确,重复性好,可作为红景天药材中红景天苷、大花红天素的测定方法。Objective To establish a determination method for the contents of salidroside and crenulatin in Rhodiolae Sacrae Herbra, and to compare the content differences of the two compounds in Rhodiolae Sacrae Herbra from different batches. Methods Using HPLC-ELSD method and Kromasil Cl8 column （250 nun × 4.6 mm, 5 μm）, with acetonitrile and water as mobile phase, gradient elution at flow rate of 1.0 mL/min, column temperature of 30 ℃, nitrogen flow rate of 2.0 L/min, drift tube temperature of 45 ℃, and gain of 2. Results The linear ofsalidroside was 0.806-8.06 pg （r = 0.999 6, n = 6）, and the average recovery rate was 100.56%. The linear of crenulatin was 2.142-21.42 μg （r = 0.999 9, n = 6）, and the average recovery rate was 100.40%. Conclusion This method is simple, and accurate, and has better reproducibility for the determination of the two compounds in Rhodiolae Sacrae Herbra. It could be used as a quantitative determination method for salidroside and crenulatin in Rhodiolae Sacrae Herbra.

关 键 词：HPLC-ELSD 红景天 红景天苷 大花红天素 质量标准 

分 类 号：R286.22[医药卫生—中药学]