二岐马先蒿苯丙素类活性成分研究  

Phenylpropanoids constituents of Pedicularis dichotoma

作　　者：褚洪标[1] 曾红[1] 梁生林[1] 梁兆昌[1] 李庆耀[1] 周秋贵[1] 

机构地区：[1]井冈山大学医学院,江西吉安343009

出　　处：《中草药》2014年第9期1223-1227,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31260084);江西省教育厅科技项目(GJJ11539)

摘　　要：目的研究二岐马先蒿Pedicularis dichotoma全草的苯丙素类化学成分及其抗疲劳活性。方法运用硅胶、反相、凝胶Sephadex LH-20等柱色谱手段进行分离纯化，根据理化性质及波谱数据鉴定化合物的结构；通过小鼠游泳训练模型评价抗疲劳活性。结果从二岐马先蒿全草95％乙醇提取物的正丁醇萃取部位分离得到12个苯丙素类化合物，分别鉴定为毛蕊花苷（1）、异毛蕊花苷（2）、米团花苷A（3）、紫地黄苷D（4）、角胡麻苷（5）、异角胡麻苷（6）、顺．角胡麻苷（7）、citrusinC（8）、robustasideB（9）、darendosideB（10）、无刺枣苄苷I（11）、红景天苷（12）。其中，化合物1能显著延长小鼠游泳时间。结论化合物l～12均为首次从该植物中分离得到，其中化合物ll为首次从马先蒿属植物中分离得到。苯丙素类化合物具有明显的抗疲劳活性。Objective To study the phenylpropanoid constituents from the whole plants ofPedicular＆ dichotoma and the anti-fatigue activities in vivo. Methods The n-BuOH fraction of 95% ethanol extract from P. dichotoma was separated and purified by silica gel, re-phase and Sephadex LH-20 column chromatography. The structures of the compounds were identified by physicochemical properties and various spectroscopic methods. The compounds were tested on anti-fatigue activity using mice in swimming model. Results Twelve phenylpropanoid compounds were isolated and purified. Their structures were identified as verbascoside （1）, isoverbascoside （2）, leucoseptoside A （3）, jionoside D （4）, martynoside （5）, isomartynoside （6）, cis-martynoside （7）, citrusin C （8）, robustaside B （9）, darendoside B （10）, zizybeoside I （11）, and salidroside （12）. Verbascoside could obviously prolong the swimming time of mice. Conclusion Compounds 1-12 are obtained from the plant for the first time, and compound 11 is reported from the plants ofPedicularis Linn. for the first time. Phenylpropanoids show obvious anti-fatigue activities.

关 键 词：二岐马先蒿 马先蒿属 苯丙素类化合物 毛蕊花苷 米团花苷A 无刺枣苄苷I 红景天苷 抗疲劳 

分 类 号：R284.2[医药卫生—中药学]