评估中药煎液络合铁离子能力的分析方法研究  

Analytical method to evaluate chelating capacity of constituents in decoction of Chinese materia medica with free iron ions

作　　者：陈巧利[1] 华瑛 王翠玲[1] 张宁[1] 孙艳妮[1] 王征[1] 刘建利[1] 

机构地区：[1]西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,陕西西安710069 [2]陕西省肿瘤医院药剂科,陕西西安710061

出　　处：《中草药》2014年第10期1402-1406,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(20872118;30070905);长江学者和创新团队发展计划资助(IRT1174)

摘　　要：目的建立体外筛选分析的方法来确定临床所用治疗阿尔茨海默病、帕金森综合症以及骨质疏松症的中药中哪种中药以及哪些成分是与铁离子（Fe3＋）络合的主要成分，并评估其络合强度。方法基于Fe3＋对螺环罗丹明B酰肼衍生物开环显色反应的催化作用，建立了催化光度法测定中药煎液中游离态Fe3＋的方法，进而通过加入一定量Fe3＋测定被络合的程度来评估中药成分与铁络合的强度。结果补肾中药对Fe3＋的络合率较高；采用催化光度法测定中药煎液中游离Fe抖的量，线性范围1．68～22．4mg／L，r=0．9990；平均加标回收率在90．45％～104．11％，RSD〈5％。结论该方法可应用于评估中药络合游离Fe3＋强度。Objective To establish the in vitro screening method for determining what kinds of Chinese materia medica （CMM） and which main constituents in the medicine used for the treatment of Alzheimer＇s disease, Parkinson＇s disease, and osteoporosis can chelate with free iron ions （Fe3＋） and evaluating the chelating strength. Methods Based on the catalytic effect of Fe3＋ on the spirolactam （nonfluorescence） to ring-open amide reaction （fluorescence）, the catalytic spectrophotometric method was established to determine the free Fe3＋ in the decoction of CMM, by adding a certain amount of iron ion and determinig the chelating level to evaluate the chelating strength of the constituents in CMM. Results The chelating strength ofkidney-tonifying CMM is stronger. The catalytic spectrophotometric method could be used to determine the free Fe3＋ in the decoction of CMM and the linearity was good in the range of 1.68--22.4 mg/L, r = 0.999 0. The average recovery was between 90.45% and 104.11% with RSD 〈 5%. Conclusion This method can be used to evaluate the chelating capacity of constituents in CMM with Fe3＋.

关 键 词：铁离子 中药 罗丹明B衍生物 催化光度法 络合能力 阿尔茨海默病 帕金森综合症 骨质疏松症 显色反应 

分 类 号：R286.02[医药卫生—中药学]