告达庭下调β-catenin/survivin抑制神经胶质瘤C6细胞迁移实验研究  

Inhibition of Caudatin on migration of glioma C6 cells in vitro by downregulating β-catenin/survivin expression

作　　者：费洪荣[1,2] 王桂玲[2] 赵莹[2] 周洪雷[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]泰山医学院药学院,山东泰安271016

出　　处：《中草药》2014年第10期1439-1443,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81272683);山东省自然科学基金资助项目(ZR2011HQ034)

摘　　要：目的观察告达庭对大鼠神经胶质瘤C6细胞增殖和迁移能力的影响,并初步探讨其内在的分子机制。方法 MTT法检测告达庭对C6细胞存活力的影响;流式细胞术检测细胞周期的变化;细胞划痕实验及Transwell迁移小室检测告达庭对C6细胞体外迁移能力的抑制作用;免疫印迹实验检测告达庭对C6细胞内β-catenin、Survivin以及细胞周期相关蛋白CyclinD1和Cdk4蛋白表达的影响。结果告达庭能够剂量依赖性地抑制C6细胞的增殖并阻滞细胞于G1期,其机制与下调细胞周期素CyclinD1和Cdk4的表达相关;告达庭给药后,C6细胞的迁移能力明显受到抑制,同时β-catenin及其下游因子Survivin的表达呈显著下降趋势。结论告达庭具有抑制C6细胞的增殖和体外迁移能力的作用,其机制可能与抑制β-catenin蛋白及Survivin的表达相关。Objective To investigate the effects of Caudatin on cell proliferation and migration of human glioma cell line C6 and the potential mechanisms. Methods Cell viability was evaluated by MTT assay, Cell cycle distribution was assessed by propidium iodide flow cytometry. Scarification test and Transwell assay were used to measure the cell migration. The effects of Caudatin on the expression of β-catenin, Survivin, CyclinD1, and Cdk4 were determined by Western blotting assay. Results Caudatin inhibited the C6 cell viability in a dose dependent manner, and caused an accumulation of C6 cells in G1 phase, and Western blotting results suggested that Caudatin inhibited the expression of CyclinD 1 and Cdk4. The migration ability of C6 cells was significantly blocked by Caudatin treatment. Caudatin could significantly down-regulate the expression of β-catenin and Survivin in C6 cells. Conclusion Caudatin could inhibit the C6 cell migration in vitro with a dose dependent way, and the potential mechanism might be related to inhibiting the expression of β-catenin and Survivin in C6 cells.

关 键 词：告达庭 神经胶质瘤 细胞迁移 细胞周期 Β-CATENIN SURVIVIN 

分 类 号：R285.5[医药卫生—中药学]