UPLC同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A  

Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in Scutellaria baicalensis by UPLC

作　　者：刘金欣[1,2] 孟繁蕴[1,2] 张胜海[3] 卢恒 周骁腾[1,2] 侯静怡[1,2] 李耿[1,4] 

机构地区：[1]北京师范大学中药资源保护与利用北京市重点实验室,北京100875 [2]北京师范大学资源学院资源生态与中药资源研究所,北京100088 [3]北京中医药大学,北京100102 [4]中日友好医院全国中西医结合心血管病中心,北京100029

出　　处：《中草药》2014年第10期1477-1480,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81072999)

摘　　要：目的建立用超高效液相色谱(UPLC)同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的方法。方法采用UPLC色谱系统,BEH C18色谱柱(50 mm×2.1 mm,1.7μm);流动相为乙腈-0.1%甲酸,梯度洗脱,体积流量为0.6 mL/min;进样量为2μL;检测波长为280 nm。结果本方法可在15 min内完成1次色谱分析,黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A色谱峰之间有良好的分离度,5种成分的浓度和各自峰面积之间有着良好的线性关系,精密度、重复性及加样回收率的RSD均小于3.0%。结论本方法快捷、准确,重复性好,能同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的量。Objective To establish a UPLC method for the simultaneous determination of five constituents （baicalin, wogonoside, baicalein, wogonin, and oroxylin-A） in Scutellaria baicalensis. Methods Five constituents in S. baicalensis were simultaneously determined by UPLC with Waters Acquity UPLC BEH Cl8 column （50 mm ×2.1mm,1.7μm）. With a gradient program of acetonitrile-0.1% formic acid at the flow rate of 0.6 mL/min, and the detection wavelength was set at 280 nm. Results Baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in S. baicalensis were clearly and respectively separated in 15 min. The relationship between the concentration and the peak areas of the five constituents in S. baicalensis was all linear. The RSD of recovery, precision, and reproducibility was all less than 3.0%. Conclusion The method is rapid, simple, reliable, and accurate, and has been successfully used to the quantification of the five constituents in S. baicalensis.

关 键 词：黄芩 黄芩苷 黄芩素 汉黄芩苷 汉黄芩素 千层纸素A 超高效液相色谱 

分 类 号：R286.2[医药卫生—中药学]