狂犬病病毒街毒株对体外培养小鼠海马神经元的损伤作用及其机制  

Injury of hippocampus neuron induced by street strain of rabies virus and its mechanism

作　　者：宋艳[1,2] 宋颖[1] 侯金利 张茂林[1] 

机构地区：[1]北华大学护理学院内科护理学教研室,吉林吉林132013 [2]吉林大学人兽共患病研究所病毒病研究室,吉林长春130062

出　　处：《吉林大学学报（医学版）》2014年第3期525-528,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(31072147)

摘　　要：目的:探讨狂犬病病毒街毒株(RV)感染神经细胞的形态学表现,揭示狂犬病病毒致神经功能异常的机制。方法:体内实验,20只C57/BL小鼠随机分为实验组和对照组,每组10只,实验组小鼠脑内接种30μL RV病毒液(10TCID50),对照组小鼠脑内接种等量细胞维持液,利用抗RV抗体检测病毒抗原在小鼠脑组织的分布。体外实验,培养原代海马神经细胞,培养1周后,感染狂犬病病毒,免疫荧光检测感染72、96和120h后病毒的增殖情况。结果:体内实验,狂犬病病毒感染4d后,在海马CA1区锥状神经元胞体和树突中均能检测到狂犬病毒抗原;狂犬病病毒感染7d后,仅在海马CA1区胞体中检测到狂犬病病毒抗原。体外实验,狂犬病RV感染体外培养的原代神经细胞120h后,感染的神经元数量最多,且感染的神经元树突数量减少。结论:狂犬病RV可感染、损伤海马CA1区树突,从而引起小鼠神经功能异常。Objective To discuss the morphological changes of neurons infected with street strain of rabies virus （RV）,and to explore the mechanism of the neuronal dysfunction caused by RV.Methods Invivo,the C57/BL mice were randomly divided into experimental group（n=10）and control group（n=10）.The mice in experimental group were inoculated intracranially at 30 μL RV suspension （10TCID50 ）, and the mice in control group were inoculated with identical volume of cell maintenance medium.Anti-RV antibody was used to detect the RV antigen distribution in brain tissue of the mice.Invitro,the primary hippocampal neurons were cultured for 1 week and the cells were infected by RV.The proliferation of RV was detected by immunofluorescence method after infection for 72,96,and 120 h.Results The RV antigen was found in neurons and neuronal dendrites of hippocampus CA1 4 d after infection;however, the RV antigen was detected only in neuronal dendrites of hippocampus CA1 7 d after infection.The number of infected neurons was the most 120 h after infection,and the number of infected dendrites was decreased.Conclusion RV can infect and injure the neuronal dendrites of hippocampus CA1 region and lead to neuronal dysfunction of the mice.

关 键 词：狂犬病病毒街毒株 神经元 神经功能异常 C57 BL小鼠 

分 类 号：R512.2[医药卫生—内科学]