电喷雾质谱法中K^＋加合离子受Cs^＋影响及在牛膝多糖分子量分布测定中的应用  

Effect of Cs+on K+Adduct Ions in Electrospray Mass Spectrometry and Its Application in Measurement of Molecular Weight Distribution for Achyranthes Bidentata Polysaccharides

作　　者：胡守刚[1] 郭寅龙[1] 吕龙[1] 田庚元[1] 

机构地区：[1]中国科学院上海有机化学研究所,

出　　处：《化学学报》2001年第3期421-425,共5页Acta Chimica Sinica

基　　金：上海市自然科学基金

摘　　要：以测定牛膝多糖的分子量分布为例，研究了在Cs+存在情况下，电喷雾(ESI)/飞行时间质谱法(TOF-MS)中出现加合离子(adduct ions)的规律.实验中发现，牛膝多糖样品中加入适量的Cs+，可影响其与Na+，K+加合离子的相对丰度，使K+加合离子成为几乎唯一的加合离子形式，增加了样品测定的灵敏度，简化了图谱，实现了对牛膝多糖数均分子量和重均分子量的测定，并获得了满意结果.另外讨论了Cs+浓度、喷管电压(nozzle potential)对这一现象的影响.Relative abundance of adduct ions was studied in measuring molecular weight distribution of achyranthes bidentata polysaccharides using electrospray ionization(ESI)/time - of - night mass spectrometry (TOF - MS) in the presence of Cs+. The relative abundance of K+ adduct ions,(C6H10O5)(n) + H2O + H+ + K+, was affected by Cs+ and became an almost unique form of adduct ions at suitable concentrations of Cs (+). This can greatly increase sensitivity and simplify the spectrum. Measuring the molecular weight distribution of achyranthes bidentata polysaccharides thus became easier, and electrospray ionization found a new application. The concentration of Cs+ and nozzle potential were key factors that affect the relative abundance of K+ adduct ions.

关 键 词：电喷雾离子化 飞行时间质谱 牛膝多糖 加合离子 中药 铯离子 分子量分布测定 钾离子 

分 类 号：R284.1[医药卫生—中药学]