Raman光谱研究铜锌超氧化物歧化酶及其金属取代衍生物的二级结构  

A Study on the Secondary Structures of Copper-Zinc Superokide Dismutase and its Metal-Substituted Derivatives by Raman Spectroscopy

作　　者：汤银燕[1] 王志林[1] 郁清[1] 耿金龙[1] 罗勤慧[1] 

机构地区：[1]南京大学配位化学研究所,南京210093

出　　处：《无机化学学报》2001年第2期263-268,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目!(No.29831010)

摘　　要：本文测定了铜锌超氧化物歧化酶(Cu2Zn2SOD)及其金属取代衍生物Cu2Ni2SOD的Raman光谱，对图谱进行了归属，并定量测定了两种SOD的二级结构，同时对结构与活性的关系进行了讨论。The Raman spectra of copper-zinc superoxide dismutase (Cu2Zn2SOD) and its metal-substituted derivative Cu2Ni2SOD were measured and the major bands were assigned. The secondary structure contents of the proteins were obtained quantitatively. The results show that the Cu2Zn2SOD contains 3.0% alpha -helix, 45.1% beta -structure and 51.9% random coil, which is in agreement with the results obtained by the X-ray crystal analysis and FTIR, and the Cu2Ni2SOD contains 8.2% alpha -helix, 27.2% beta -structure and 64.1% random coil. It can be concluded that the activity of SOD decreases with the decreasing of beta -structure and increasing of random coil.

关 键 词：铜锌超氧化物歧化酶 RAMAN光谱 二级结构 金属取代衍生物 活性 

分 类 号：Q554.9[生物学—生物化学]