光度法测定指甲和人发中微量锌  

PHOTOMETRIC DETERMINATION OF MICROAMOUNTS OF ZINC IN HUMAN HAIR AND NAILS

作　　者：周磊[1] 杜登学[1] 刘耘[1] 

机构地区：[1]山东轻工业学院化工系,济南250100

出　　处：《理化检验（化学分册）》2001年第2期62-63,65,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：研究了在溴化十六烷基三甲基铵和曲通 X- 1 0 0存在下 ,锌与 2 - (3,5-二溴 - 2 -吡啶偶氮 ) - 5-二乙氨基苯酚的显色反应。结果表明 ,在 p H7.0～ 9.5范围内 ,锌与 2 - (3,5-二溴 - 2 -吡啶偶氮 ) - 5-二乙氨基苯酚形成 1∶ 2稳定配合物 ,其最大吸收峰位于 572 nm处 ,摩尔吸光系数为 1 .3×1 0 5L·mol-1·cm-1。锌含量在 0～ 1 2 .0 μg/2 5ml范围内符合比耳定律。方法用于测定指甲和人发中微量锌 ,结果满意。The colour reaction of zinc(Ⅱ) with 3,5 diBr-PADAP in the presence of CTMAB and Triton X 100 is studied. The complex with its molar ratio of 1 to 2 is formed in a pH 7.0~9.5 buffer solution. The absorption maxima lies at 572nm with its apparent molar absorptivity of 1.3×10 5L·mol -1 ·cm -1 . Beer′s law is obeyed in the range of 0~12.0μg/25ml for zinc(Ⅱ). The method has been applied to determine microamounts of zinc in human hair and nails with satisfactory results.

关 键 词：光度法 锌 表面活性剂 指甲 人发 微量元素 分析 

分 类 号：Q581[生物学—生物化学] O657.3[理学—分析化学]