同源四倍体盾叶薯蓣遗传稳定性研究  

Genetic Stability Study on Autotetraploid Plant of Dioscorea zingiberensis

作　　者：黄和平[1,2] 高山林[3] 黄璐琦[2] 汪电雷[1] 黄鹏[1] 

机构地区：[1]安徽中医药大学,安徽合肥230031 [2]中国中医科学院,北京100700 [3]中国药科大学,江苏南京210009

出　　处：《中药材》2014年第3期365-368,共4页Journal of Chinese Medicinal Materials

基　　金：安徽省高等学校自然科学研究重点项目(KJ2011A191);国家地方联合安徽济人中药提取工程研究中心开放基金(2012gczx02);安徽省中药材良种繁育工程技术研究中心开放基金(2012lzfy01)

摘　　要：目的:考察盾叶薯蓣同源四倍体的遗传稳定性。方法:利用显微方法进行根尖染色体鉴定;于生长稳定期观测田间苗的农艺性状;测定蛋白质含量并进行蛋白质聚丙烯酰胺凝胶电泳;测定和比较薯蓣皂苷元含量。结果:盾叶薯蓣四倍体株系的染色体数目仍为40,农艺性状保持了器官巨型性的多倍体特征,各株系蛋白质电泳谱带基本相似,薯蓣皂苷元及蛋白质含量均高于二倍体对照株系。结论:本实验证实人工诱导的盾叶薯蓣同源四倍体具有较好的遗传稳定性,为后续的多倍体优良品种选育工作奠定了基础。Objective：To study the genetic stability of autotetraploid plant of Dioscorea zingiberensis. Methods：The chromosome of root-tip was determined by photomicroscope,and the agronomic characters were observed in the period of stable growth. The protein con- tent was determined and the experiment of protein polyacrylamide gel electrophoresis was carried out. Furthemore, the diosgenin content was determined and compared. Results ： The chromosome number of autotetraploid plantlet was 2n = 4x = 40. The agronomic characters showed typical autotetraploid characteristics. The contents of diosgenin and protein of autotetraploid were higher than that of the diploid. The protein electrophoresis bands of all the lines were similar. Conclusion ： The experiment confirmed that the autotetraploid plant of Dioscorea zingiberensis, which was artificially induced, had good genetic stability. It lays the foundation for the polyploid breeding to develop superior varieties of Dioscorca zingiberensis.

关 键 词：盾叶薯蓣 同源四倍体 遗传稳定性 

分 类 号：R282.2[医药卫生—中药学]