黄连须生物碱时空分布及产地加工工艺研究  

Effect of Different Parts,Harvesting Time and Processing Technologies on Alkaloids Content of Coptis chinensis Adventitious Root

作　　者：庞婕[1] 王德珍[1] 邹宗尧 王燕枝[1] 高倩[1] 李学刚[1] 

机构地区：[1]西南大学药学院,重庆400716

出　　处：《中药材》2014年第3期395-397,共3页Journal of Chinese Medicinal Materials

基　　金：科技部支撑计划项目(2011BAI13B02-1);2013年重庆市"百名工程技术高端人才培养计划"

摘　　要：目的：研究黄连须生物碱含量的时空分布,以及黄连须产地加工工艺。方法：采用高效液相色谱法测定不同采收时间、不同生长部位黄连须中生物碱含量,并研究了水洗对黄连须鲜品和干品生物碱含量的影响。结果：不同采收时间的黄连须总生物碱含量基本不变,为2.5%-2.9%。其中,小檗碱和黄连碱含量较高,达1%左右,巴马汀含量很低,仅0.1%;靠近黄连主根的黄连须（近须）总生物碱含量较高,近4%,远离主根（远须）的总生物碱含量较低,为2%左右;黄连须鲜品洗涤后生物碱含量几乎不变,干品洗涤后生物碱大大减少。结论：灰渣中的近须的利用价值大于丢弃在田间的远须;水洗对黄连须鲜品生物碱含量没有影响。Objective：To investigate the effect of different parts, harvesting time and processing technologies on alkaloids content of Coptis chinensis adventitious root. Methods：The content of alkaloids were analyzed by HPLC. Results：The content of total alkaloids in adventitious root harvested in different time was ranged from 2.5% to 2. 9% ,in which that of berberine and coptisine were the highest, reaching to 1% , and that of palmatine was only 0. 1%. It suggested there was no significant difference of total alkaloids at different harvesting time. Nevertheless, the difference of the alkaloids content from different parts was much significant. The content of total alkaloid of adventitious root near to rhizome was about 4% ,2 times higher than that away from rhizome （only 2% ）. In addition, different process- ing technologies would affect alkaloids content obviously. There was hardly loss of alkaloids when the fresh adventitious root was washed with water,but it would decrease alkaloids content when the dried adventitious root was washed. Conclusion：Medicine value of Coptis chinensis adventitious root near to rhizome is higher than that away from rhizome. And fresh Coptis chinensis adventitious root can be washed with water.

关 键 词：黄连须 生物碱含量 时空分布 加工工艺 

分 类 号：R282.4[医药卫生—中药学]