三种提取方法结合GC-MS分析比较草豆蔻的挥发性成分  

GC-MS Analysis for Volatile Components from Alpiniae Katsumadai Semen by Three Extraction Methods

作　　者：杨彬彬[1] 容蓉[1] 胡金芳[1] 杨勇[1] 吕青涛[1] 蒋海强[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中药材》2014年第3期443-447,共5页Journal of Chinese Medicinal Materials

基　　金：国家重点基础研究发展计划(973计划)(2007CB512601);山东省高等学校科技计划项目(J10LF20)

摘　　要：目的:分析草豆蔻的挥发性成分。方法:分别采用水蒸气蒸馏法、顶空进样法以及超临界CO2萃取法提取草豆蔻中的挥发性成分,采用气相色谱-质谱技术(GC-MS)结合Kovat's保留指数进行检测分析。结果:水蒸气蒸馏法和顶空进样法检测到草豆蔻挥发性成分主要为萜类化合物,而超临界CO2萃取物中挥发性成分主要为烯类、醇类及芳香类化合物。结论:不同的样品前处理方法分别侧重于药材中不同类型的挥发性成分;利用GC-MS分析技术并结合Kovat's保留指数进行定性分析更准确、快速。Objective：To analysis the volatile components in Alpiniae Katsumadai Semen. Methods：The volatile components were extracted from Alpiniae Katsumadai Semen by steam distillation, head space injection and supercritieal fluid extraction respectively, and then analyzed by GC-MS combined with Kovat＇s retention index. Resuhs ：The volatile components extracted by steam distillation or head space extraction were found more likely to be terpenoids, whereas components extracted by supercritical fluid extraction were more likely to be alkenes,alcohols and aromatic compounds. Conclusion：Different sample pre-treatment methods are focused on different types of volatile components;Identification of the volatile components by GC-MS combined with Kovat＇s retention index is more accurate and rapid.

关 键 词：草豆蔻 GC-MS 水蒸气蒸馏 超临界CO2萃取 顶空进样 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]