柱前衍生化HPLC法测定蚯蚓中还原型谷胱甘肽(GSH)和半胱氨酸(CySH)  

Determination of Reduced Glutathione(GSH) and Cystein(CySH) in Earthworm by HPLC with Pre-Colum Derivatization

作　　者：胡北[1] 张小勇[1] 沈丽[1] 崔胜云[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《化学试剂》2014年第6期537-540,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(21165021)

摘　　要：利用5,5'-二硫双-2-硝基苯甲酸(DTNB)为衍生化试剂,采用细胞破壁、衍生化为一体的同步衍生化提取方法,利用高效液相色谱法(HPLC)分别测定了蚯蚓中GSH、CySH含量。3次测定蚯蚓中GSH和CySH含量均值分别为0.632 2和0.860 0μmol/g,测定的加标回收率均值依次为100.8%和99.05%,检测限分别达5.43和4.96μmol/L。利用本方法可实现简便、选择性测定蚯蚓中GSH和CySH的含量。GSH and CySH in earthworm have been determined by HPLC with semultaneous derivatizations during cell rupture using DTNB as derivatizing agent. The average amounts of GSH and CySH were 0. 632 2 μmol/g and 0. 860 0 μmol/g, respectively. The recoveries of the methods were 100. 8%, 99.05% with the detection limits of 5.43 and 4.96 μmol/L, respectively. The methods can be used to determine of the contents of GSH and CySH in earthworm.

关 键 词：5 5′-二硫双-2-硝基苯甲酸 衍生化 高效液相色谱法 巯基化合物 

分 类 号：O657.39[理学—分析化学] O657.32[理学—化学]