棉蚜化学感受蛋白AgosCSP2的基因克隆、时空表达动态及气味结合特征分析  

Gene Cloning,Temporal Expression Analysis and Binding Characteristics of Chemosensory Protein AgosCSP2 in Aphis gossypii(Glover)

作　　者：李娜 张帅[2] 雒珺瑜[2] 吕丽敏[2] 崔金杰[2] 李国清[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095 [2]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2014年第3期228-236,共9页Cotton Science

基　　金："十二五"国家科技支撑计划项目:棉花病虫害生态控制技术研究与集成示范(2012BAD19B05)

摘　　要：化学感受蛋白是一类存在于昆虫化学感受器中的可溶性蛋白,可能与昆虫识别外界化学信息有关。本研究从棉蚜(Aphis gossypii)转录组数据中克隆得到1条编码化学感受蛋白的cDNA序列,命名为AgosCSP2(GenBank登录号KC017751)。使用qRT-PCR技术对此化学感受蛋白mRNA在棉蚜中的时空表达谱进行了分析,结果表明AgosCSP2在多数类型棉蚜若虫中的表达量明显高于成虫中的表达量;在寄主为棉花时的表达量高于寄主为木槿时;在无翅成蚜中足的表达量相对其他组织较高。荧光竞争性结合试验表明AgosCSP2与气味化合物的结合能力较弱。推测该基因主要参与棉蚜若虫对寄主的适应过程,具有嗅觉以外的生理功能。试验结果为进一步研究AgosCSP2的功能奠定了基础。Chemosensory proteins（CSPs）,which belongs to a class of small soluble polypeptides in insect chemical receptors,may be related with the process of storing and releasing the chemical odors for insects.In this study,one full-length cDNA of chemosensory protein gene was cloned from Aphis gossypii,named AgosCSP2 （GenBank accession No.KC017751）.The temporal expression pattern of the gene was studied by qRT-PCR analysis.The results indicated that the expression levels in nymph were significantly higher than those in adult; the AgosCSP2 was higher expressed in the host of cotton than that in the overwintering host hibiscus and it was higher expressed in leg than that in other organizations.The binding characteristics showed that AgosCSP2 had low binding abilities with odorant ligands.The result suggested that the AgosCSP2 gene most likely functioned in the process of cotton aphid nymph adaptation to host,but not functioned as the chemosensory of the insect.This experiment may be valuable for studying the role of AgosCSP2 in A.gossypii chemical sensors.

关 键 词：棉蚜 化学感受蛋白 表达谱 原核表达 纯化 荧光竞争结合试验 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治]