黔产金樱子变型的ISSR分析  

ISSR Analysis on Rosa laevigata Michx. from Different Variants

作　　者：王晓多 王晓玲[2] 王晓丽[3] 

机构地区：[1]贵阳市花溪区农业局,贵州贵阳550025 [2]贵州大学,贵州贵阳550025 [3]贵阳医学院药用植物与生药学教研室,贵州贵阳550004

出　　处：《安徽农业科学》2014年第17期5425-5426,共2页Journal of Anhui Agricultural Sciences

基　　金：黔产大宗药材金樱子;苦参质量评价研究。黔科合ZY字[2012]3004号

摘　　要：[目的]揭示贵州境内不同变型金樱子10个居群间的遗传变异情况。[方法]采用ISSR分子标记技术，从ISSR随机引物中，筛选有效引物，进行扩增，用NTSYSpc，ver．2．02软件进行UPGMA聚类分析。[结果]10个居群不同变型金樱子的相似性系数在0．09～0．35，聚成A、B2大类。[结论]同一变型的金樱子样品并不严格为一类，不同变型金樱子样品之间的遗传变异较大。[ Objective ] The heritable variation of 10 was uncovered for different variants of Rosa laevigata Michx. in Guizhou. [ Method ] By u- sing ISSR molecular marker technology, effective primers were screened from random primers, and were amplified by ISSR. UPGMA cluster analysis was conducted by NTSYSpc, ver. 2.02. [ Result] The similarity coefficients of the 10 populations were from 0.09 to 0. 35, and divided into 2 groups for A and B. [ Conclusion] Individuals of the same population did not strictly cluster together and the genetic diversity of individuals in different populations was considerably high.

关 键 词：金樱子 ISSR 遗传变异 

分 类 号：S567[农业科学—中草药栽培]