酶促乙酯化和分子蒸馏联用制备高DHA乙酯的工艺  

Preparation of ethyl esters highly enriched in DHA via enzymatic ethylation and molecular distillation

作　　者：孙兆敏[1] 张芹[1] 郭正霞[1] 王静凤[1] 李兆杰[1] 薛勇[1] 王玉明[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2014年第12期167-171,共5页Science and Technology of Food Industry

基　　金："泰山学者"建设工程专项经费;长江学者和创新团队发展计划资助;海洋公益性行业专项(201105029);国际科技合作项目(2010DFA31330);山东省科技发展计划(2012G0021506)

摘　　要：利用固定化脂肪酶催化乙酯化反应将裂殖壶菌总脂转化为乙酯,并借助分子蒸馏手段制备得到了高DHA含量的乙酯产品。实验考察了反应温度、时间、底物质量配比和脂肪酶添加量等参数对酶促乙酯化反应的影响。在较优条件下(反应温度40℃,反应时间24h,底物质量比10∶2,Lipozyme 435添加量为油脂质量的6%),固定化脂肪酶的操作稳定性良好。采用分子蒸馏技术富集酶促醇解所得裂殖壶菌乙酯中的DHA,可得到DHA含量高于80%的乙酯产品,通过多级分子蒸馏和将轻相组分回流的方式可以显著提高DHA含量乙酯的得率。In the present study,the total lipids from Schizochytrium sp. were ethylated by immobilized lipase and ethyl esters highly enriched in C22：6 n-3（DHA） was obtained through molecular distillation. The effect of temperature, reaction time, substrate ratio, and enzyme dosage on enzymatic ethylation was investigated. Under optimum reaction conditions（reaction temperature 40℃,reaction time 24h,substrate ratio 10：2,Lipozyme 435 dosage 6%）,immobilized lipase had good operational stability. Ethyl esters with no less than 80% DHA was obtained via molecular distillation,and the yield of DHA ethyl esters could be significantly improved by multi- stage molecular distillation and recycle of light fractions.

关 键 词：裂殖壶菌 乙酯化 分子蒸馏 DHA 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程]