2型糖尿病大鼠胰岛β细胞PDX-1基因表达及GLP-1的干预作用  

Effect of GLP-1 on the expression of PDX-1 gene in islet beta cells of type 2 diabetes rats

作　　者：吴美芬[1] 陈晓铭[1] 武革[1] 郑坤杰[1] 潘海燕[2] 方烁[1] 

机构地区：[1]广东医学院附属医院内分泌科,广东省湛江市524001 [2]广东医学院,广东省东莞市523808

出　　处：《实用医学杂志》2014年第11期1699-1701,共3页The Journal of Practical Medicine

基　　金：广东省科技计划资助项目(编号:2011B031800231);广东省医学科研基金资助项目(编号:B2011243);广东医学院青年基金项目(编号:2010XQ112)

摘　　要：目的:观察胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)类似物利拉鲁肽对2型糖尿病大鼠胰腺十二指肠同源盒-1(pancreatic duodenal homeobox-1,PDX-1)基因表达水平的影响。方法:将60只雄性SD大鼠平均分为3组:正常对照组、糖尿病未治疗组及GLP-1组,正常对照组给予普通饲料喂养,糖尿病未治疗组与GLP-1组给予高脂饲料喂养,于第8周末以链脲佐菌素(Streptozotocin,STZ)30 mg/(kg·bw)腹腔注射高脂饲料喂养组,成模后GLP-1组给予利拉鲁肽200μg/kg皮下注射,每日2次共持续8周。其余2组予等量的生理盐水皮下注射。以实时荧光定量逆转录-聚合酶链反应(Real-Time fluorescent quantitative reverse transcription-polymerase chain reaction,Real-Time RT-PCR)法检测大鼠胰腺胰岛细胞PDX-1基因的表达。结果:与正常对照组比较,GLP-1组及未治疗组PDX-1 mRNA的表达量明显减低(P<0.001),GLP-1治疗后2型糖尿病大鼠胰腺PDX-1 mRNA的表达量可明显增高(P<0.001)。结论:GLP-1可上调2型糖尿病大鼠胰岛β细胞PDX-1基因的表达。Objective To observe the effect of liraglutide, an analogue of glucagon like peptide -1 （GLP-1）, on the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats. Methods Sixty male SD rats were divided into 3 groups： normal control group, diabetic model group and GLP-1 group. Normal control group was given normal diet and diabetic model group and GLP-1 group were given high fat diet. After the eighth weekend, the high fat diet group was intraperitoneally injected with streptozotocin （STZ） 30 mg/（kg ·bw）, and the GLP-1 group was subcutaneously injected with liraglutide 200 μg/kg with 2 times per day for 8 weeks. The other groups were treated with normal saline by subcutaneous injection. Real-time fluorescent quantitative reverse transcription polymerase chain reaction （Real-Time RT-PCR） was used to detect the expression of PDX-1 gene in rat pancreatic islet cells. Results The expressions of PDX-1 mRNA in the GLP-1 group and untreated group were significantly lower （P 〈 0.001 ） than that in the normal control group, but significantly higher than that after GLP-ltreatment （P 〈 0.001 ）. Conclusion GLP-1 can upregulate the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats.

关 键 词：糖尿病 2型 大鼠 PDX-1 GLP-1 利拉鲁肽 

分 类 号：R587.1[医药卫生—内分泌]