酪氨酸激酶Src在白色念珠菌感染中的机制研究  

Effects of tyrosine kinase Src during C.albicans infection in murine macrophage

作　　者：崔树娜[1] 钱静[1] 卜平[1] 

机构地区：[1]扬州大学医学院中西医结合学系,225001

出　　处：《实用医学杂志》2014年第11期1702-1704,共3页The Journal of Practical Medicine

基　　金：江苏省高校自然科学研究项目(编号:13KJB310022);中国博士后基金项目(编号:2013M541741);江苏省中医药局科技项目(编号:LZ13248);扬州大学高层次人才科研启动基金项目(2012)(编号:13KJB310022)

摘　　要：目的：探讨酪氨酸激酶Src在白色念珠菌感染中的作用及其机制。方法：通过Alarmarblue法检测不同浓度Src抑制剂PP2作用巨噬细胞不同时间点对细胞增殖的影响，通过荧光定量法检测巨噬细胞对FITC标记的白色念珠菌感染吞噬作用的影响．采用ELISA法检测PP2对巨噬细胞感染白色念珠菌产生细胞因子TNF-α和IL-10的影响。结果：0～33．3μmol／L的PP2作用细胞2h后对细胞生长无明显影响。11．1、33．3μmol／L的PP2作用细胞24h后细胞增殖率分别为78％、9％，48h后增殖率分别为54％、13％。11．1μmol／L的PP2仅在作用巨噬细胞48h后，抑制巨噬细胞对白色念珠菌的吞噬，此作用可能与抑制增殖相关．然而，PP2能显著抑制白色念珠菌对巨噬细胞的免疫应答，表现为显著抑制细胞因子TNF—α和IL-10的产生．有效浓度为11．1～33．3μmol／L（P〈0．01）。结论：酪氨酸激酶Src在巨噬细胞识别白色念珠菌感染中起着重要作用，尤其在细胞因子免疫应答中作用更加显著。Objective To investigate the role of tyrosine kinase Src in a murine C.a!bicans infection model. Methods Observed cell proliferation by alarmarblue assay at 2, 24 and 48 h after Src inhibitor PP2 treatment. Phagocytosis was determined by a fluorometric assay. Cytokine TNF-α and IL-10 production was detected by ELISA. Results The 0 ~ 33.3 μmol/L PP2 had no effect on cell proliferation after PP2 treatment for 2 h. When the PP2 treatment extended to 24 or 48 h, PP2 （11.1, 33.3 μmol/L） showed significant inhibition on cell proliferation with 78%, 9%, and 54%, 13%, respectively. At 48 h after 11.1 μmol/L PP2 treatment, the internalization of C.albicans in macrophage is significantly inhibited, contributing to the inhibition of cell proliferation. However, the 11.1 and 33.3 μmol/L PP2 significantly inhibited the cytokine TNF-α and IL-10 production during C.albicons infection （P 〈 0.01 ）. Conclusion Src kinase played an important role during C.albicans infection, especially for the cytokine TNF-α and IL- 10 production.

关 键 词：念珠菌病 SRC激酶 白色念珠菌 巨噬细胞 

分 类 号：R379.4[医药卫生—病原生物学]