姜黄素对糖尿病痛性周围神经病变大鼠脊髓炎症因子的影响  

Effect of curcumin on spinal inflammatory factor in rats with diabetic neuropathy

作　　者：张文璇[1] 赵伟成[2] 廖美娟[1] 杨承祥[2] 

机构地区：[1]徐州医学院,江苏省麻醉学重点实验室,221004 [2]广东省佛山市第一人民医院麻醉科,528000

出　　处：《实用医学杂志》2014年第11期1709-1711,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金项目(编号:S2011010003506)

摘　　要：目的：评价姜黄素对糖尿病痛性周围神经病变（DPN）大鼠脊髓炎症因子的影响。方法：腹腔注射1％链脲佐茵素（STZ）60mg／kg诱导大鼠DPN模型，在造模成功的大鼠中取20只随机分为糖尿病组（D组，n=10）和姜黄素治疗组（C组，n=10），另取10只同月龄大鼠为空白对照组（N组，n=10）。C、D组在第28天后分别每天给予200mg／kg的姜黄素和等剂量生理盐水灌胃2周。于注射STZ前（T1）、注射STZ后2、7、14、21、28、35、42d（T2—8）测定尾静脉血糖浓度；于T1、T3—8时测定大鼠右后爪缩足机械反应阎值（PWT）；于T8时处死大鼠，取腰段脊髓检测TNF-α和IL-6的含量。结果：与N组比较，D和C组T2．8时血糖浓度升高（P〈0．05），T4～8时PWT降低（P〈0．01）；与D组比较，C组T7、8时PWT升高（P〈0．05）；与T1时比较，D和C组T2～8时血糖浓度升高（P〈0．05）；与T6时比较，T7、8时C组PWT升高（P〈0．05）。与N组比较，D和C组大鼠脊髓TNF．仅和IL-6含量升高（P〈0．05）；与D组比较，C组大鼠脊髓TNF-α和IL-6的含量均下降（P〈0．05）。结论：姜黄素改善DPN大鼠的疼痛，其机制可能与降低脊髓炎症因子有关。Objective To evaluate the effect of curcumin on spinal inflammatory factor in rats with diabetic neuropathy. Methods Diabetic neuropathy was induced by intraperitoneal injection with 1% STZ （60 mg/kg） in sprague-dawley rats. These diabetic rats were randomly allocated to diabetic group （D group, n = 10） and curcumin group （C group, n = 10）. Another 10 age-matched normal rats served as controlled group （N group, n = 10）. 28 days after STZ injection, the rats in C group received daily intragastric administration of curcumin （200 mg/kg） whereas those in D group received the same volume of normal saline for 2 weeks. Caudal vein blood glucose levels at T1 （ before STZ injection ）and at T2-T8 （2,7,14,21,28,35,42 days after STZ injection）from all rats were detected. Responses to the mechanical stimulus were measured with yon Frey filament, and paw withdraw threshold （PWT） was recorded at T1 and at T3 to T8. At T8, the rats were killed and lumbar segments of spinal cord were removed to detect TNF-α and IL-6 content. Results Compared to N group, rats in both C and D group showed hyperglycemia at T2 to T8 （P 〈 0.05） and lower PWT at T4 - 8（P 〈 0.01）. Compared to D group, C group showed higher PWT at T7,8（P 〈 0.05）. Both D and C group showed higher levels of blood sugar at T2 - 8 than that at TI （P 〈 0.05）. C group showed higher PWT at T7,8 than that at T6 （P 〈 0.05）. Compared to N group, spinal TNF-α and IL-6 content increased in both D and C groups （P 〈 0.05）. Compared to D group, C group had reduction of TNF-α and IL-6 concentration （P 〈 0.05）. Conclusion Curcumin can attenuate diabetic neuropathic pain on rats probably by reducing inflammatory factor in spinal cord.

关 键 词：糖尿病周围神经病变 姜黄素 脊髓 炎症因子 

分 类 号：R285.5[医药卫生—中药学]