滞育及冷暴露对白纹伊蚊卵Hsp70基因表达的影响  

Effects of diapause and cold exposure on expression of Hsp70 in Aedes albopictus eggs

作　　者：李玉伟[1] 鲁亮[1] 刘起勇[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所媒介生物控制室,世界卫生组织媒介生物监测与管理合作中心,传染病预防控制国家重点实验室,北京102206

出　　处：《中国媒介生物学及控制杂志》2014年第3期211-214,共4页Chinese Journal of Vector Biology and Control

基　　金：国家重大科学研究计划(973项目)(2012CB955501)~~

摘　　要：目的研究白纹伊蚊卵在滞育以及冷暴露条件下,热休克蛋白70基因(Hsp70)的表达情况,探究Hsp70在增强白纹伊蚊卵耐寒性方面的作用。方法以无冷暴露的非滞育卵作为对照组,采用RT.qPCR方法检测白纹伊蚊滞育卵和非滞育卵Hsp70 mRNA的表达情况。结果室温25℃条件下,滞育卵与非滞育卵表达的Hsp70差异无统计学意义(P=0.392)。与对照组相比,冷暴露后蚊卵Hsp70表达量均增加,且滞育卵Hsp70的表达量明显高于非滞育卵的表达量,分别是其对照组的16.44和5.22倍。结论Hsp70并不是白纹伊蚊滞育期间的重要组成部分,但是在冷暴露的恢复期表达量上调。Objective To investigate the expression of Hsp70 in Aedes albopictus diapausing and nondiapausing eggs after cold exposure and to determine the role of Hsp70 in enhancing cold tolerance of Ae. albopictus eggs. Methods Hsp70 mRNA expression in Ae. albopictus diapausing and nondiapausing eggs after cold exposure was determined by real-time quantitative PCR, using non-cold-exposed nondiapausing eggs as a control. Results No significant difference in Hsp70 mRNA expression was observed between diapausing and nondiapausing eggs at room temperature （25 ℃ ） （P=0.392）. Hsp70 mRNA expression increased in both diapausing and nondiapausing eggs after cold exposure, as compared with that of the control. Furthermore, the diapausing eggs had a higher amount of Hsp70 mRNA than the nondiapausing eggs; the expression levels in diapausing and nondiapausing eggs were 16.44 and 5.22 times, respectively, that of the control. Conclusion The expression of Hsp70 is usually not up-regulated during diapausing process, but it is up-regulated during recovery from cold exposure.

关 键 词：白纹伊蚊 卵 冷暴露 滞育 热休克蛋白70基因 

分 类 号：R384.1[医药卫生—医学寄生虫学]