德国小蠊虫体挥发物成分分析  

Component analysis of volatile attractants from Blattella germanica

作　　者：邱文毅 文平[2,3] 吴海磊[4] 何德雨 郭峰琦 莫建初[3] 钱进 

机构地区：[1]常州出入境检验检疫局,江苏常州213003 [2]江苏农用激素工程技术研究中心,江苏常州213022 [3]浙江大学昆虫科学研究所,浙江杭州310058 [4]南京出入境检验检疫局

出　　处：《中国媒介生物学及控制杂志》2014年第3期235-238,共4页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局科技计划项目(2012IK229)~~

摘　　要：目的查明德国小蠊自身挥发物的成分及其引诱活性。方法使用顶空吹扫固相微萃取（HS-SPME）提取德国小蠊挥发物，并通过气相-质谱（GC-MS）联用分析SPME提取物中的主要成分，使用气相一触角电位（GC-EAD）来分析电生理活性的主要组分，并使用有活性的组分进行诱捕实验。结果发现德国小蠊挥发物中包含至少19个组分，其中含量较高的13个组分分别是一些C2～C5的挥发性醇和酸。用标准化合物样品所做的GC-EAD分析结果表明，仅6种挥发羧酸对德国小蠊具有明显的触角电位反应。现场诱捕实验证实，酸类物质混配后对德国小蠊具有一定的引诱效果。结论德国小蠊释放有活性的挥发物并可起到一定引诱作用，引起群体聚集。Objective To determine the chemical property and attractive activity of volatiles from Blattella germanica. Methods Volatiles from B. gerrnanica were collected by headspace SPME method in purge flow. GC-MS was used to identify the main components in SPME extracts. GC-EAD was used to identify electrophysiologically active components. Active components were used for trapping test. Results Analysis of the extracts after SPME revealed that there were at least 19 components in the volatiles, with 13 volatile C2-C5 carboxylic acids and alcohols as main components. GC-EAD test with standard chemicals showed that only 6 volatile earboxylic acids elicited electroantennogram responses. Field trapping test showed that the mixture of 6 volatile carboxylic acids were effective in attracting B. germanica. Conclusion Attractive volatiles emitted by B. germanica play a role in colony aggregation.

关 键 词：德国小蠊 挥发物 触角电位反应 

分 类 号：R384.9[医药卫生—医学寄生虫学]