基于高通量测序技术的连作杨树人工林土壤细菌多样性研究  

Soil bacteria diversity in continuous cropping poplar plantation by high throughput sequencing

作　　者：韩亚飞[1] 伊文慧 王文波[1] 王延平[1] 王华田[1] 

机构地区：[1]山东省高校森林培育重点实验室、国家林业局泰山森林生态系统定位研究站、山东农业大学林学院,山东泰安271018

出　　处：《山东大学学报（理学版）》2014年第5期1-6,共6页Journal of Shandong University(Natural Science)

基　　金：国家重点基础研究发展计划(“九七三”计划)子专题资助项目(2012CB416904/zgc);国家自然科学基金资助项目(31070550,31270670)

摘　　要：土壤细菌在土壤微生物中占有重要地位,在土壤环境中具有十分重要的生态功能。为了了解杨树人工林根际和非根际土壤的细菌群落结构,明确连作对杨树土壤细菌多样性的影响,采用454焦磷酸测序技术对连作杨树人工林根际土壤和非根际土壤细菌群落多样性进行分析。结果表明,根际土壤共得到了3 036个OTU,非根际土壤共得到了3 051个OTU,两组样品共同包含的OTU共有1 404个。两组土壤的共有细菌群落有12个,其中Proteobacteria、Acidobacteria、Actinobacteria为优势菌群。从丰富度指数Chao1和多样性指数Shannon角度分析,发现连作导致根际土壤和非根际土壤细菌的丰富度和多样性都有所下降,且根际土壤细菌群落的改变更为显著。Soil bacteria,which have significant ecological functions in soil environment,play a very important role in soil biogeochemical processes. In this study,454 pyrosequencing was applied to investigate the bacterial diversity of soil samples from rhizosphere（ RS）and non-rhizosphere（ NRS）in a continuous cropping poplar plantation（ Populus × eu-ramericana‘Neva’）in order to explore the soil bacterial community structure of poplar plantation and reveal the effects of continuous cropping on soil bacterial diversity. The results indicated that a total of 3 036 operational taxonomic units （ OTUs）were obtained from RS samples and 3 051 from NRS samples,1 404 OTUs were shared by two groups of sam-ples. The bacteria in soils from rhizosphere and non-rhizosphere belong to Acidobacteria,Actinobacteria,Armatimona-detes,Bacteroidetes,Chloroflexi,Gemmatimonadetes,Nitrospira,Planctomycetes,Proteobacteria,Firmicutes,TM7 and unclassified-bacteria,and the bacteria of Proteobacteria,Acidobacteria and Actinobacteria were the dominant groups. The indices of Chao1 and Shannon showed that continuous cropping descended the population diversities and enrichment of bacteria in soil samples from both rhizosphere and non-rhizosphere,and the descend intent was much higher in rhizosphere than in non-rhizosphere.

关 键 词：连作人工林 根际和非根际土壤 细菌群落多样性 高通量测序 

分 类 号：S718.8[农业科学—林学]