右心导管法测大鼠肺动脉压力的改良  

The Improvement of Right Heart Catheterization Method to Measure Pulmonary Artery Pressure in Rats

作　　者：陈传斯[1] 庞玉生[1] 马建法[1] 韦宇明 王凯[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021 [2]广西卫生职业技术学院,广西南宁530021

出　　处：《现代生物医学进展》2014年第15期2863-2866,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81160040)

摘　　要：目的：目前常用的测量大鼠肺动脉压力的右心导管法存在一定的缺陷，且很难得到典型的压力曲线图。本实验对大鼠经颈外静脉插管与测压的方法进行改良，同时与已有报道的实验结果进行比较，并提供正常SD大鼠右心房、右心室及肺动脉的压力参考值及典型的压力曲线图，以协助研究人员判断导管位置，及时调整导管的深度和方向，快速测出肺动脉压力。方法：雌雄不分的清洁级SD大鼠共30只，体重180-230g，6～7周龄。应用自制的末端呈一弧形的PE-10管，采用改良后的右心导管法，经颈外静脉插入大鼠心腔及肺动脉，检测并计算大鼠右心房、右心室和肺动脉的收缩压、舒张压及肺动脉平均压。结果：右心房压力波动较平缓，呈小波浪形；右心室压力曲线波动大，骤升骤降；肺动脉压力曲线有重搏波。正常SD大鼠右心房舒张压为（2．03±2．56）mmHg，收缩压为（2．82±1．85）mmHg；右心室舒张压为（5．72±3．99）mmHg，收缩压为（18．73±4．80）mmHg；肺动脉舒张压为（15．27±2．64）mmHg，收缩压为（18．49±2．53）mmHg，肺动脉平均压为（16．34±2．32）mmHg。右心室收缩压与肺动脉收缩压无明显差异（P〉0．05）。结论：改良后的方法可准确到达大鼠肺动脉，提供的压力参考值及曲线图有助于研究人员顺利完成测压实验。Objective： There are some flaws in the right heart catheterization and it is difficult to get a typical pressure curve. The purpose of this experiment is to improve the conventional right heart catheterization method to measure pulmonary artery pressure in rats, and provide normal Sprague-Dawley rats＇ reference pressure values and the pressure curves to help researchers determine the position of the catheter and the depth of the catheter. Methods： 30 male or female Sprague-Dawley rats （weight 180-230 g, age 6-7 weeks ）. The end of PE-10 tube was made a curved, which was similar with the arc formed of right ventricle and pulmonary artery. All the rats were performed right heart catheterization in the external jugular vein. Then, the systolic pressure and the diastolic pressure of right atrium, right ventricle and pulmonary artery were detected, and mean pressure was calculated by the method that mean pressure=2/3 diastolic pressure ＋1/3 systolic pressure. Results： Right atrium pressure was flat, such as small waves, but right ventricular pressure curve became surges and sags, and the pulmonary artery pressure curve had dicrotic waves. The right atrium diastolic pressure of normal Sprague-Dawley rat was （2.03± 2.56） mmHg, the right atrium systolic pressure was （2.82± 1.85） mmHg; The right ventricular diastolic pressure was （5.72 ± 3.99） mmHg, the right ventricular systolic pressure was （18.73 ± 4.80） mmHg; The diastolic pulmonary artery pressure was （15.27 ± 2.64） mmHg, the systolic pulmonary artery pressure was （18.49 ± 2.53） mmHg and the mean pulmonary artery pressure was （16.34 ± 2.32） mmHg. There was no significant difference between right ventrieular systolic pressure and systolic pulmonary artery pressure （P 〉0.05）. Conclusions： This method can reach the pulmonary artery accurately, and the pressure values and graphs help researchers successfully complete experiment.

关 键 词：右心导管法 肺动脉压 压力曲线 

分 类 号：Q95-3[生物学—动物学] R331[医药卫生—人体生理学]