Ras蛋白在地塞米松体外诱导大鼠胚胎垂体生长激素细胞分化过程中的作用  

The effect of Ras on Dexamethasone-induced Rat Embryonic Pituitary Somatotroph Cell Differentiation in vitro

作　　者：程玉[1] 张宇[1] 胡志强[2] 李国忠[1] 项莹[3] 胡恩喜[1] 王超[4] 关峰[2] 陶宇[1] 郑宏山[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001 [2]北京世纪坛医院神经外科,北京100038 [3]哈尔滨医科大学附属第二医院内分泌与代谢科,黑龙江哈尔滨150086 [4]哈尔滨医科大学附属第四医院神经外科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2014年第17期3228-3231,3279,共5页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅基金项目(11513053)

摘　　要：目的:探究Ras蛋白在地塞米松体外诱导大鼠胚胎垂体生长激素细胞分化过程中的作用。方法:本课题利用大鼠胚胎垂体细胞的无血清原代细胞培养技术,在地塞米松诱导生长激素细胞分化的过程中,加入蛋白Ras的抑制剂Manumycin,利用免疫荧光、western-blot、放射免疫分析和MTT等技术对Ras蛋白在糖皮质激素体外诱导生长激素细胞分化中的作用进行研究。结果:地塞米松能够显著提高生长激素阳性细胞百分比和生长激素的含量(P<0.01)。加入不同浓度的Manumycin后,地塞米松诱导的生长激素阳性细胞百分比显著降低(P<0.01),生长激素的含量亦出现降低(P<0.05)。结论:Ras蛋白在地塞米松诱导垂体生长激素细胞分化过程中发挥重要作用。Objective： The function of Ras is to be explored in dexamethasone-induced differentiation of rat embryonic pituitary somatotroph cells in vitro. Methods： The pituitary was harvested for fetal pituitary cell suspension. In the experiment Ras inhibitor Manumycin of different concentrations were added to the suspension for pretreatment and cells were cultured with a medium containing Dex. And also immunofluorescence, Western-blot, radioimmtmoassay and MTT Technology were used in this experiment. Results： Dex treatment increased the percentage of GH-positive cells and GH content （P〈0.01）. After pretreatment with Manumycin, the percentage of Dex-induced GH-positive cells decreased significantly （P〈0.01）, and the GH content also reduced （P〈0.05）. Conclusion： Ras protein plays an important role in the dexamethasone-induced growth hormone cell differentiation.

关 键 词：地塞米松 垂体 生长激素细胞 分4L RAS蛋白 

分 类 号：Q95-3[生物学—动物学] Q593.2