醒脑液对PAP双转基因痴呆小鼠行为学及胆碱能系统的早期干预作用说  

Early Intervention of Xingnaoye Decoction on Behavioral Science and Related Enzymes of Cholinergic System Towards PAP Double Transgenic Dementia Mice

作　　者：常富业 单媛莉 张云岭[2] 王新祥[2] 郑宏[2] 马涛[2] 刘雪梅[2] 阎妍[2] 

机构地区：[1]煤炭总医院老年病科,北京100028 [2]北京中医药大学东方医院实验中心,北京100078

出　　处：《中华中医药学刊》2014年第6期1258-1261,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81072851)

摘　　要：目的:观察醒脑液早期干预对阿尔茨海默病模型APPswe/PS△E9双转基因小鼠行为学及脑中胆碱能系统相关酶的影响。方法:将75只PAP双转基因小鼠随机随机分为空白对照组(模型组)、阳性对照组、用药高剂量组、用药中剂量组和用药低剂量组,每组各15只。15只遗传背景相同的转基因阴性的小鼠作为正常动物组。所试药物稀释至相同体积灌胃给药,正常动物组及空白对照组给以等体积蒸馏水灌胃,连续灌胃4个月。采用Morris水迷宫测定小鼠空间学习记忆能力,避暗箱测定其条件学习记忆能力。使用比色法检测小鼠脑中乙酰胆碱(Ach)、乙酰胆碱转移酶(ChAT)及乙酰胆碱酯酶(AchE)的活性。结果:与正常组比较,模型组小鼠的学习记忆能力均有所下降,脑组织中Ach、ChAT、AchE的含量均下降。与模型组比较,阳性对照组、用药高剂量组及用药中剂量组小鼠血浆中Ach含量均有所提高,而AchE含量则出现下降。结论:醒脑液能改善阿尔茨海默病模型小鼠的学习记忆能力,其作用机制可能与调控胆碱能系统有关。Objective ： To observe the effect of Xingnaoye decoction on behavioral science and related enzymes of cholinergie system in brain towards early intervention in Alzheimer＇s disease model APParel PS △E9 double transgenic mice. Method ：75 PAP double transgenie mouse were randomly divided into blank control group, positive control group, high dose group ,medium dose group and low dose group（ model group） ,with 15 mouse in each group. 15 transgenic negative mouse of the same genetic background as the normal control group. The Xingnaoye decoction was diluted to the same volume for three dose groups, and normal control group and blank control group were given an equal volume of distilled water, orally for 4 months. Using the Morris water maze to determine learning and memory ability of mice in space, and avoid black - box to determine one in condition. The eolorimetric determination activity of acetylcholine （ Aeh ）, acetylcholine transferase （ ChAT ） and acetylcholinesterase （ Ache ） in brain for mice. Result： Compared with the normal group,it was decreased that the learning and memory ability of mice and the contents of Ach, ChAT , AchE in model group mouse. Compared with the model group, the content of Ach in positive control group, high dose group and medium dose group was increased, while the content of AchE was decreased. Conclusion：The Xingnaoye decoction can improve the learning and memory ability of Alzheimer＇s disease model mice model of Alzheimer＇s disease on, and the mechanism may be related to regulation of cholinergic system.

关 键 词：醒脑液 阿尔茨海默病 转基因小鼠 乙酰胆碱酯酶 乙酰胆碱转移酶 乙酰胆碱 

分 类 号：R258.5[医药卫生—中医内科学]