心血瘀阻证心肌微环境基质金属蛋白酶-2,9表达的实验研究  

Expressions of Matrix Metalloproteinase-2 And,9 Iin cariacHeart Blood Stasis Syndrome of Myocardial Microenvironment

作　　者：郑景辉[1,2] 杨恒宝 宁桂兰[1] 陈建军[1] 刘华盛[1] 吴发胜[1] 李勇华[3] 简维雄[2] 王丽萍[2] 

机构地区：[1]广西中医药大学附属瑞康医院心血管内科,广西南宁530011 [2]湖南中医药大学中医诊断学国家重点学科,国家中医药管理局病理生理实验室,湖南长沙410007 [3]重庆三峡医药高等专科学校,重庆404120

出　　处：《中华中医药学刊》2014年第6期1290-1292,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81102595);广西自然科学基金项目(2012GXNSFAA053113);广西壮族自治区中医药民族医药科研(GZZC1223)

摘　　要：目的:观察基质金属蛋白酶-2、9在心血瘀阻证心肌微环境的变化。方法:建立大鼠急性心梗心血瘀阻证模型,分为假手术组、健康对照组、心虚瘀阻证组,然后用ELISA方法观测其基质金属蛋白酶2、9的表达。结果:基质金属蛋白酶2血瘀阻证表达高于健康对照和假手术组(P<0.05),基质金属蛋白酶9三组间均有差异,其中心血瘀阻证组最高,其次为假手术组、健康对照组(P<0.05)。结论:基质金属蛋白酶-2、9参与心血瘀阻证心肌微环境的变化,是中医证候本质的物质学基础。Objective ： To observe the expressions of matrix metalloproteinase - 2 and , 9 in cariac heart blood stasis syndrome of myocardial microenvironment mieroenvironment. Method ： the The acute myocardial infarction rats of blood stasis models were established. Besides, there were with the sham operation group, model group, guilty stasis group, and then use the ELISA method to observe the expressions of matrix metalloproteinase 2,9. Result：The expressions of matrix metalloproteinase - 2 was higher in blood stasis group than that in healthy control and sham operation group （ P 〈 0.05 ）. The expression of matrix metalloproteinase metalloproteinase - 9 was differences the there groups, in which the heart blood stasis syndrome groups was the highest, followed by the expression of falsesham operation group, and healthy control group （ P 〈 0. 05 ）. Conclusion ： The matrix metalloproteinase - 2/,9 contrubut to the change of blood stasis syndrome of myocardial microenvironment, and are the basis of TCM syndrome essence of material science.

关 键 词：心血瘀阻证 微环境 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R285.8[医药卫生—中药学]