喜树碱衍生物SN-38对人白血病细胞系HL-60增殖及凋亡作用的实验研究  

Experimental Study on Effect of SN-38 on Apoptosis and Proliferation of Human Leukemia Cell Lines HL-60

作　　者：封蔚莹[1] 宋春娇[1] 张志坚[1] 钟永根[1] 

机构地区：[1]绍兴市人民医院 浙江大学绍兴医院,浙江绍兴312000

出　　处：《中华中医药学刊》2014年第6期1406-1408,I0011,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2012ZB159);绍兴市公益性应用计划项目(2012B70066)

摘　　要：目的:观察喜树碱衍生物SN-38对人白血病细胞系HL-60细胞的增殖抑制和诱导凋亡作用,并探讨其可能的作用机制。方法:采用CCK法检测SN-38对HL-60细胞的增殖抑制作用;采用流式细胞术检测细胞凋亡率;特异性荧光底物检测Caspase-3、Caspase-8、Caspase-9活性;观察不同种类Caspase抑制剂对SN-38诱导HL-60细胞的增殖和凋亡作用的影响。结果:CCK法显示SN-38明显抑制HL-60细胞增殖,流式细胞凋亡分析及Caspase抑制剂研究,显示SN-38通过内源性Caspase信号通路诱导HL-60细胞凋亡。结论:SN-38通过内源性Caspase信号通路抑制HL-60细胞增殖和诱导凋亡。Objective ： To research the inhibition of SN - 38 on the growth of HL - 60 cell in vitro. Methods ： This experiment was adopted with CCK assay for the inhibition of SN - 38 on the growth of HL - 60. The apoptotic cells were detected by flow cytometry. The activity of Caspase - 3, Caspase - 8 and Caspase - 9 was determined by using the specific fluorogenic substrates. Effects of Caspase inhibitors on SN - 38 - induced cytotoxicity and apoptosis were observed on HL - 60 cell. Results ： CCK assay revealed the proliferation ratio of HL - 60 was inhibited after SN - 38 treatment. The flow cytometry experiment and Caspase inhibitor research revealed that SN -38 induced apoptosis of HL -60 cell by endogenous Caspase path- way. Conclusion：SN -38 can inhibit proliferation and induce apoptosis of HL -60 cell by endogenous Caspase pathway.

关 键 词：SN-38 白血病 

分 类 号：R284[医药卫生—中药学]