新型单羰基姜黄素衍生物B06对不同辐射抗拒鼻咽癌细胞的抑制作用  

Inhibition effect of curcumin mono-carbonyl derivative B06 on nasop-haryngeal carcinoma cells with different radioresistance

作　　者：谢珂[1,2] 龚梁[1,2] 陈建强[1,2] 邹坚定[1,2] 鲁杰[1,2] 熊华[1,2] 施雪莉[1,2] 

机构地区：[1]浙江省慈溪市人民医院 [2]温州医科大学附属慈溪医院耳鼻咽喉科,浙江慈溪315300

出　　处：《中国现代医生》2014年第17期21-24,共4页China Modern Doctor

基　　金：浙江省自然科学基金(LY12H13002);浙江省医药卫生科技计划项目(2011KYA145);浙江省中医药科学研究基金(2013ZB115);浙江省宁波市第二批科技项目(2012A610205;2012A610206);浙江省中医药重点研究计划(2012ZZ012);浙江省慈溪市民生类科技计划(CN2013013)

摘　　要：目的研究姜黄素及其单羰基结构衍生物B06对不同辐射抗拒鼻咽癌细胞株CNE-2与CNE-2R的作用。方法以MTT法检测姜黄素和B06对细胞增殖的影响,以流式细胞仪检测姜黄素和B06对细胞凋亡的影响,以Western-blot检测姜黄素和B06对ERS通路相关蛋白的影响。结果姜黄素对CNE-2和CNE-2R的IC50分别为8.35μM和6.84μM,B06对CNE-2和CNE-2R的IC50分别为1.09μM和0.74μM。B06能激活CNE-2R细胞ERS通路中关键蛋白CHOP、XBP-1、ATF-4的表达,而姜黄素则不能激活其表达。结论新型单羰基姜黄素衍生物B06能更显著地抑制辐射抗拒鼻咽癌细胞的增殖,该作用机制很可能是通过ERS通路发挥,提示B06具有抑制辐射抗拒鼻咽癌细胞的潜在价值。Objective To study the effects of B06, a mono-carbonyl derivative of curcumin, on homologous nasopharyngeal carcinoma cell lines CNE-2R and CNE-2 with different radioresistance. Methods The effects of B06 and curcumin on cell proliferation were determined by MTF assay. The cell apoptosis was determined by flow cytometry. The effects of B06 and curcumin on ERS related proteins were determined by western-blot. Results Curcumin inhibited CNE-2 and CNE-2R ceils with IC50 of 8.35 μM and 6.84μM respectively. B06 inhibited CNE-2 and CNE-2R cell lines with ICS0 of 1.09 μM and 0.74 μM respectively. B06 could activate the expression of CHOP, XBP-1 and ATF-4, the key proteins of ERS passway, while curcumin could not. Conclusion The new mono-carbonyl curcumin derivative B06 can suppress the proliferation of nasopharyngeal carcinoma cells with radioresistance more significantly. The mechanism of this effect is probably through the ERS pathway, suggesting that B06 has the potential value to inhibit nasopharytlgeal carcinoma cells with radioresistance.

关 键 词：姜黄素 单羰基衍生物 鼻咽癌 辐射抗拒 

分 类 号：R285.5[医药卫生—中药学]