免疫酶染色法检测心肌炎患者柯萨奇B组病毒特异性IgM抗体的研究  

Detection of COX-B virus specific IgM in patients with myocarditis by enzyme immunoassay

作　　者：胡开华[1] 邱练芬[1] 唐英蓉[2] 

机构地区：[1]四川省人民医院检验科,成都610071 [2]四川省人民医院心内科,成都610071

出　　处：《医学科技》2000年第2期11-14,共4页

基　　金：卫生厅下达资助

摘　　要：目的:研究早期诊断成人病毒性心肌炎柯萨奇 B 组病毒 IgM 抗体(CBVIgM)的检测方法。方法:用免疫酶染色法(酶染玻片法)测定患者血清 CBVIgM,并与病毒分离和微量细胞中和抗体检测相比较。结果:129例住院心肌炎患者血清 CBVIgM 阳性82例(63.5%)、46例心肌炎咽拭子分离出 CBV12例,(其 CBVIgM 阳性11例),病毒分离患者血清(双份26例、单份20例)其 CBVIgM 阳性者32例,中和抗体≥4倍升高和单份血清≥1∶320者共27例。40例健康对照组病毒分离和中和抗体均阴性,CBVIgM 阳性2例。结论:酶染玻片法检测 CBVIgM,方法简单、快速、可靠,其结果与对照组相比有非常显著差异,与病毒分离阳性有91.6%相符,与血清中和抗体阳性有84.3%相符。Objective:To establish a method of enzyme immunoassay for detecting serum CBV IgMin patients with myocarditis for early diagnosis of myocardilis.Method:To measure serum CBV IgM inpatients with myocarditis by enzyme immunoassay and to compare with the neutralization test of viral isola-tion.Results:82 out of 129 patients with myocarditis are positive of serum CBV IgM(63.5%).CBVhas been isolated in 12 our of 46 patients with myocarditis from their pharyngeal samples,and 11 out of12 patients isolated CBV are positive of CBV IgM,in addition to 32 out of 46 patients with myocarditisare positive of CBV IgM(acute phase serum and convalescent phase serum 26,and only acute phase su-rum 20).Twenty-seven patients are the neutralization test≥fourfold or titer of acute phase serum≥1∶320.Forty healther in control are negative in the viral isolation and in their neutralization test,only 2persons are CBV detected IgM.Conclusion:Enzyme immunoassay is a simple,rapid and accurate de-tection method of CBV IgM.There is a significant difference between the patient and control group andcompare with viral isolation and the neutralization test,the identical rates of positive are respectively91.6% and 84.3%。

关 键 词：心肌炎 柯萨奇B组病毒IgM 免疫酶染色法 

分 类 号：R542.210.3[医药卫生—心血管疾病]