干旱胁迫对银柴胡生长及生理生化特性的影响  

Effect of drought stress on growth and physiological-biochemical characteristics of Stellaria dichotoma

作　　者：郎多勇[1] 崔佳佳[2] 周达[3] 李月彤 周丽[3,4,5,6,7] 张新慧[3,4,5,6,7] 

机构地区：[1]宁夏医科大学实验动物中心,宁夏银川750004 [2]甘肃农业大学农学院,甘肃兰州730070 [3]宁夏医科大学药学院,宁夏银川750004 [4]宁夏回药现代化工程技术研究中心,宁夏银川750004 [5]宁夏回医药协同创新中心,宁夏银川750004 [6]宁夏医科大学回医药现代化省部共建教育部重点实验室,宁夏银川750004 [7]宁夏医药研究所,宁夏银川750004

出　　处：《中国中药杂志》2014年第11期1995-1999,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81202868)

摘　　要：采用盆栽试验，设置土壤饱和含水量的90％～100％，80％～90％，60％～70％，40％。50％，20％～30％5个供水水平，研究水分胁迫对银柴胡生长及生理生化特性的影响。结果表明，随着土壤含水量的降低，银柴胡株高和地上部干重逐渐下降，而根长和根干重均表现为先升高后降低的趋势。随着土壤含水量的降低，脯氨酸含量先下降后明显升高，可溶性蛋白质含量逐渐下降；保护酶SOD和POD均表现为逐渐降低的趋势，CAT表现为先不变后降低的趋势；银柴胡叶片膜透性呈现出逐渐下降的趋势，而MDA含量呈现出先下降后上升的趋势。综上所述，在适度的干旱胁迫下银柴胡可以保持一定的抗渗透胁迫能力和清除活性氧的能力，进而保持膜的稳定。A pot experiment was conducted to study effect of drought stress on leaf physiological characteristics and growth of one year old Stellaria dichotoma seedlings. The result showed that plant height and shoot dry weight significantly decreased with decrease in soil water content;however, root length and root dry weight increased at light drought stress and decreased at severe drought stress. The result also showed that with the decrease of soil water content, proline content in S. dichotoma leaves decreased then increase, while solube protein content decreased. Activities of SOD and POD in S. dichotoma leaves significantly decreased as soil water content decreased, while activity of CAT significanlty decreased at severe drought stress. Membrane permeability in S. dichotoma leaves increased, while MDA content decreased then increased as soil water decreased. These results suggest that S. dichotoma had osmotic stress resistance ability and reactive oxygen scavenging capacity at light drought stress, which caused S. dichotoma growth was no inhibited at a certain extent drought stress.

关 键 词：银柴胡 干旱胁迫 生长 保护酶 膜脂过氧化 

分 类 号：R282[医药卫生—中药学]