芍药苷人工抗原的合成与鉴定  

Synthesis and identification of artificial antigens of paneoniflorin

作　　者：屈会化[1,2] 赵妍[3,2] 苏歆[3,2] 贺娜娜[4,2] 孙晔[3,2] 孔慧[3,2] 赵琰[3,2] 王庆国[3,2] 

机构地区：[1]北京中医药大学科研实验中心,北京100029 [2]北京中医药大学"经典方剂的应用基础研究"创新团队,北京100029 [3]北京中医药大学基础医学院,北京100029 [4]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2014年第11期2043-2046,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373542;81274043)

摘　　要：利用高碘酸钠法合成芍药苷免疫抗原PF-BSA和包被抗原PF-OVA,并通过紫外光谱检测芍药苷成功与BSA/OVA偶联。小鼠经PF-BSA免疫后,体内可产生抗芍药苷抗体,酶联免疫分析法分析证明其免疫小鼠所得抗体效价达1∶12 800,半数抑制浓度为0.791 mg·L-1,且与黄芩苷等9种中药小分子交叉反应极低。芍药苷人工抗原的合成及抗体的获得为建立ELISA和研制免疫检测试剂盒奠定了基础。Oxidation method with sodium iodide was used to synthesize immunogenic antigen （PF-BSA） and coating antigen （PF-OVA） of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA, the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer （1：12 800） and specificity （IC50=0.791 mg·L^-1）of the antiserum from mice injected with PF-BSA. Also, the antiserum showed low cross activities against nine traditional Chinese medicine （TCM） of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared, which provides the experimental basis for the further study of ELISA and its kit.

关 键 词：芍药苷 人工抗原 高碘酸钠氧化法 免疫分析方法 抗芍药苷抗体 

分 类 号：R282[医药卫生—中药学] R446.6[医药卫生—中医学]