UPLC同时测定白及药材中9种指标成分的含量  

Simultaneous determination of nine chemical markers of Bletillae Rhizoma by ultra performance liquid chromatography

作　　者：王爱民[1] 鄢艳[1,2] 兰波[1] 廖尚高[1] 王永林[3] 李勇军[1] 

机构地区：[1]贵阳医学院药学院民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [2]贵州省人民医院药剂科,贵州贵阳550001 [3]贵阳医学院贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《中国中药杂志》2014年第11期2051-2055,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81360636);贵州省中药现代化科技产业研究开发专项项目(黔科合中药字[2010]5027号);贵阳市科学技术计划项目(筑科合同[2012204]20号)

摘　　要：采用超高液相色谱（UPLC）的方法，以Acquity BEH C18色谱柱（2.1 mm×150 mm，1.7 μm），流动相为乙腈-水，梯度洗脱，流速为0.3 mL·min^-1，柱温45℃，检测波长280 nm的色谱条件同时测定白及药材中的4-羟基苯基-葡萄糖苷、4-（葡糖糖氧基）-肉桂酸葡萄糖氧基苄酯、1，4-二 [4-（葡萄糖氧）苄基]-2-异丁基苹果酸酯-2-葡萄糖苷、1，4-二[4-（葡萄糖氧）苄基]-2-异丁基苹果酸酯、二氢菲5和1，4-二[4-（葡萄糖氧）苄基]-2-异丁基苹果酸酯-2-[4-O-肉桂酰基]葡萄糖苷、二氢菲1、苄基氢菲3和1，4-二[4-（葡萄糖氧）苄基]-2-异丁基苹果酸酯-2-[4-O-肉桂酰基-6-O-乙酰基]葡萄糖苷9个指标成分。各指标成分之间分离度良好，在选定的浓度范围内线性关系良好（r≥0.999 3），平均加样回收率（n=6）为98.15%～102.2%，RSD为2.1%～3.6%，表明所建立的方法重复性、耐用性良好，结果准确，经对野生和人工种植白及药材分析，野生白及所测定的9种成分含量高于人工栽培白及药材，为白及药材的质量评价和控制提供科学依据。A UPLC method has been developed in the current investigation for simultaneous determination of nine chemical markers of Bletilla striata,4-hydroxymethylphenyl β-D-glucoside,blestroside,dactylorhin A,militarine,dihydrophenanthrene 5, gymnoside V,dihydrophenanthrene 1,benzylphenanthrene 3 and gymnosides Ⅸ. Separation was performed at 45℃ on an ACQUITY UPLC BEH C18 column （2.1 mm×150 mm, 1.7 μm） with a gradient solvent system of acetonitrile-water as the mobile phase. The flow rate was 0.3 mL·min^-1, the detection wavelength was 280 nm. The results showed that the nine chemical markers could be well resolved and that in the selected linear range, all calibration curves of the nine chemical markers showed good linearity （r≥0.999 3）. The recoveries （n=6） were in the range of 98.15%～102.2% and RSDs were between 2.1%～3.6%. The data suggested that the developed UPLC-UV method had good reproducibility, robustness, and accuracy, which was suitable for the quality control of Bletilla striata. Applications of the method showed that the nine chemical markers had higher contents in the wild B. striata than in the cultivated ones.

关 键 词：白及 多指标成分 超高液相色谱(UPLC) 

分 类 号：R284.1[医药卫生—中药学]