高效液相色谱法测定人体血中氯喹含量  

CHLOROQUINE CONTENTS IN HUMAN BLOOD DETECTED BY HPLC

作　　者：欧阳华学[1] 康万民[2] 付成平[1] 刘群华[2] 杨锡孟 高斌[2] 张媛春 魏继炳[2] 张富南[2] 

机构地区：[1]四川省农业科学院分析测试中心,四川成都610066 [2]四川省寄生虫病防治研究所,四川成都610041 [3]云南省元江县人民医院,云南元江653300 [4]玉溪市卫生防疫站,云南玉溪653100

出　　处：《实用寄生虫病杂志》2000年第4期154-156,共3页Journal of Practical Parasitic Diseases

基　　金：四川省科委和省卫生厅97-92资助

摘　　要：为了深入了解氯喹药代动力学有关情况 ,用高效液相色谱检测 4名志愿者口服氯喹总量分别为 6 0 0mg、6 0 0mg、90 0mg和 12 0 0mg后 ,血药浓度 16 8h后尚有 141μg/L、134μg/L、2 0 8μg/L和 2 98μg/L ,均明显高于有效杀灭疟原虫 2 0 μg/L浓度。用单剂 6 0 0mg和首日 6 0 0mg ,次日 30 0mg氯喹治疗间日疟现症病人各 1例 ,追踪观察 2 8h均无症状复发和原虫再现 ,获得痊愈。结果表明口服氯喹在人体内清除非常缓慢 ,维持有效杀灭疟原虫时间长 ,是治疗间日疟、三日疟和卵型疟的首选药。The contents of chloroquine in blood were determined by HPLC in 4 volunteers taking orally 600mg, 600mg, 900mg, and 1200mg of chloroquine respectively 168 hours after administration, the concentration were 141μg/L, 134μg/L, 208μg/L, and 298 μg/L, which were obviously higher than the effective level (20μg/L) of chloroquine against Plasmodium Two acute cases with vivax malaria were experimentally treated with a single dose of 600mg, or 300mg, 600mg in 2 days of chloroquine No relapse of symptoms or parasite presented during 28 days' follow up study The results showed that the chloroquine could maintain effective concentration in blood for relatively a long time and which would provide scientific basis for re assessment of malaria treatment program

关 键 词：氯喹 高效液相色谱 药代动力学 间日疟 疟疾 

分 类 号：R531.305[医药卫生—内科学] R978.61[医药卫生—临床医学]