基于UPLC-Q-TOF/MS研究参附注射液的物质基础  

Material basis of Shenfu injection based on UPLC-Q-TOF/MS

作　　者：何家乐[1,2] 周思思[1] 马增春[2] 梁乾德[2] 王宇光[2] 谭洪玲[2] 肖成荣[2] 汤响林[2] 高月[2] 

机构地区：[1]安徽医科大学研究生学院,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国药理学通报》2014年第3期133-137,共5页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2012CB518402;2011CB505304);国家自然科学基金资助项目(No 81274127)

摘　　要：目的对参附注射液中的人参皂苷和生物碱成分进行定性,并且对参附注射液中主要成分在大鼠体内的药物动力学过程进行研究。方法运用LC-MS对参附注射液中的成分进行定性,并测定大鼠血清中多种人参皂苷的浓度,计算其药动学参数。结果参附注射液中定性出16种人参皂苷成分和10种生物碱成分。人参皂苷Rd、Rg1、Rb1、Ro、Rc、Rb2的T12α分别为0.32±0.25、0.11±0.04、0.30±0.24、0.11±0.02、0.24±0.19、0.23±0.12 h,人参皂苷Rd、Rb1、Rc、Rb2的T12β分别10.25±0.39、21.31±3.64、13.95±2.56、15.06±1.54 h。结论该方法简便、灵敏、特异,适用于血清中6种人参皂苷成分的药物动力学研究;人参皂苷Rg1、Ro在大鼠体内的分布消除速度较快,Rd、Rb1、Rc、Rb2分布消除较慢。参附注射液体外、体内成分的分析为组分配伍研究建立了可靠的方法。Aim To qualitativly analyze the ginsen-osides and alkaloids and study the pharmaeokineties of main composition in Shenfu injection in rsts. Methods The ginsenosides and alkaloids in Shenfu injection were analyzed qualitatively by the established LC-MS methods, and simultaneously the concentrations of gin- senoside Rd, Rg1 , Rbl, Ro, Rc, Rb2 in serum were determined and the pharmacokinetic parameters were calculated. Result 16 ginsenosides and 10 alkaloids were detected in Shetrfu injection, T1/2α of Rd, Rg1, Rb1, Ro, Rc, Rbe were 0.32±0.25, 0.11 ±0.04, 0.30 ±0.24, 0.11±0.02, 0.24 ± 0,19, 0.23 ± 0. 12h respectively, T1/2β were 10.25 ±0. 39, 21.31± 3.64, 13.95 ± 2. 56, 15.06 ± 1.54 h respectively. Conclusions The method is simple, sensitive, spe- cific and suitable for the pharmacokinetic study of 6 ginsenosides in serum. The distribution and elimination of Rg1 and Ro are rapid, while those of Rd, Rbl, Rc, and Rb2 are slow. The study of Shenfu injection in vitro and vivo has established a reliable method for compo- nent compatibility.

关 键 词：参附注射液 人参皂苷 生物碱 药动学 液质联用 组分配伍 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]