双偶氮染料刚果红共振瑞利散射法测大观霉素  

Resonance rayleigh scattering method for the determination of spectinomycin with bisazo dye congo red

作　　者：谭建红[1] 邱明凤 江虹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《化学研究与应用》2014年第3期457-459,共3页Chemical Research and Application

基　　金：重庆市教委科技基金项目(KJ121321)资助

摘　　要：建立了测定痕量大观霉素的共振瑞利散射法。在pH 3．25的Tris-盐酸缓冲介质中，大观霉素与酸性双偶氮染料刚果红相互作用后使体系的共振瑞利散射强度（ΔIRRS ）明显增强，在370 nm处的ΔIRRS最大。增强的共振瑞利散射强度ΔIRRS与0．015～0．33 mg·L-1范围的大观霉素呈良好的线性关系，方法的检出限（3Sb/S）为0．012mg·L-1。该法用于市售大观霉素药物及人尿中大观霉素含量的测定，结果满意。To develop a novel resonance rayleigh scattering method for determination of trace spectinomycin. In pH 3. 25 Tris-HCl buffer medium,the resonance rayleigh scattering intensity of acidic bisazo dye congo red was greatly enhanced when spectinomycin was added. And the strongestΔIRRS was located 370 nm. The scattering intensity（△IRRS ） was directly proportional to the mass con-centration of spectinomycin in the range of 0. 015～0. 33 mg·L-1 with a determination limit（3Sb/S）was 0. 012 mg·L-1. The method has been applied to the determination of spectinomycin in the human urine and commercially available spectinomycin medicament with satisfactory results.

关 键 词：双偶氮染料 刚果红 共振瑞利散射 大观霉素 

分 类 号：O657.3[理学—分析化学]