鳡肌肉生长抑制素基因内含子和部分启动子序列的克隆与分析  

Cloning and Analysis of the Myostatin Introns and Promoter in Elopichthys bambusa

作　　者：郁建锋[1] 顾志良[1] 邵芳[1] 冯晓夏[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《常熟理工学院学报》2014年第2期17-22,共6页Journal of Changshu Institute of Technology

基　　金：农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金"鳡鱼肌肉生长抑制素基因的克隆;表达和遗传变异研究"(ky2009047)

摘　　要：采用PCR和染色体步移法克隆了鳡（Elopichthys bambusa）肌肉生长抑制素长789 bp的内含子1、987 bp的内含子2及长662 bp的部分上游启动子序列.同源性比对分析表明，鳡MSTN的内含子1、2与鲤科鱼类的同源性相对较高（内含子1：60.42%～67.21%；内含子2：40.00%～51.21%），与其他鱼类、鸟类以及哺乳类动物的同源性则较低.生物信息学方法预测鳡MSTN的部分启动子序列中含有2个近转录起始点的TBP（TATA框）、2个CTF（CAAT框）、3个Sp1、10个C/EBP、4个Oct1、2个潜在AP1的结合位点，此外还存在1个GATA、3个HNF1、1个NF-κB和1个CREB等多种转录因子结合位点以及3个E-框.The objective of this study is to clone the sequences of myostatin gene introns and its promoter in Elopichthys bambusa. PCR and Genome Walking were used to acquire two introns and promoter of the mostatin gene. Two introns and a partial sequence of the myostatin promoter were cloned, of which the lengths were 789 bp, 987 bp and 622 bp, respectively. Bioinformatics analysis showed that the sequences of the two myostatin in-trons of Elopichthys bambusa had higher similarity to the myostatin introns of other cyprinidae fish （intron 1：60.42%-67.21%, intron 2： 40.00%-51.21%） than the other animals′. The nucleotide sequence analysis showed that the partial sequence of the myostatin promoter contained several potential regulatory factors binding sites, of which are two TBP, two CTF, three Sp1, ten C/EBP, four Oct1, two AP1, one GATA, three HNF, one NF-κB, one CREB, three E-box and so on.

关 键 词：鳡 肌肉生长抑制素 内含子 启动子 

分 类 号：S917.4[农业科学—水产科学]