核桃青皮提取物对食管癌细胞增殖的抑制作用及其分子机制的探讨  

Suppression Effect on Proliferation of Esophageal Cancer Cell with Extracts of Walnut Green Seedcase

作　　者：张仲卫[1,2] 鄢文强[1] 李彩霞[2] 王冬滨[2] 李志刚[2] 司传领[3] 孔棣[2] 

机构地区：[1]天津医科大学,天津300070 [2]天津市南开医院,天津300100 [3]天津科技大学,天津300222

出　　处：《中国中西医结合外科杂志》2014年第1期43-46,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局国医大师吴咸中院士传承工作室建设项目

摘　　要：目的:观察核桃青皮提取物对人食管癌细胞增殖的抑制作用。方法:用MTT法分别测定核桃青皮粗提物和核桃青皮提取物没食子酸、乙酸乙酯、紫杉叶素、7-D-芹菜糖-儿茶酚对食管癌细胞EC9706和KYSE150的增殖抑制率,并用Western Blot检测不同药物处理后食管癌细胞中细胞周期相关蛋白CyclinD1的蛋白表达。结果:核桃青皮粗提物、没食子酸和乙酸乙酯能抑制食管癌细胞的增殖,其作用呈明显剂量依赖性,当药物浓度增加至80μg/mL时,对KYSE150的抑制率分别为82.75%、90.51%、88.36%,对EC9706的抑制率分别为85.54%、87.21%、85.17%。与对照组相比,3种药物均会显著抑制KYSE150和EC9706细胞中CyclinD1的表达。结论:核桃青皮提取物对食管癌细胞系的增殖有较强的抑制作用,其机制可能与引起细胞周期阻滞有关。Objective To observe the suppression effect of the extract of walnut green seedcase on the pro-liferation of esophageal cancer cell. Methods The suppression ratios of proliferation of esophageal cancer cellincluding EC9706 and KYSE150 caused by the extracts of walnut green seedcase, were measured using theMTT method respectively. The drugs derived from the extracts of walnut green seedcase were crude extract, gal-lic acid, EtoAC, taxifolin, and catechin-7-o-apiose. The expression of Cell cycle-related protein CyclinD1 inesophageal cancer cells was treated with different drugs by Western blotting. Results The crude extract, gal-lic acid and EtoAC had the suppression effect on the proliferation of esophageal cancer cells. The effects weredose-dependent significantly. When increasing the concentration to 80 μg/ mL, KYSE150 inhibition rates were82.75%, 90.51%, and 88.36% respectively, and EC9706 inhibition rates were 85.54%,87.21%, and 85.17% re-spectively. Compared with the control group, these three drugs would significantly inhibited the expression of Cy-clinD1. Conclusion Some of the extracts of walnut green seedcase have the obvious suppression effect on theproliferation of esophageal cancer cells, which is associated with cell cycle retardation.

关 键 词：食管癌 EC9706 KYSE150 核桃青皮提取物 细胞增殖 

分 类 号：Q95-33[生物学—动物学] R735.1[医药卫生—肿瘤]