利用微卫星标记对绵羊进行亲子鉴定  

Microsatellite DNA markers for paternity test of sheep

作　　者：刘龙腾[1] 贾春旸 努尔力.阿不力孜 张文祥[1] 赵宗胜[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《江苏农业学报》2014年第1期135-139,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31160444)

摘　　要：采用微卫星DNA分子标记方法,通过对132只父系半同胞中国美利奴羊在BM4621、BM143、OarHH55、OarJMP8、BM6438、BM6506、BM1824、OarDB6、ILSTS0049个微卫星位点的分析,计算出了中国美利奴羊在这9个位点的等位基因频率及其分布规律,并对其中7组亲子进行了亲子鉴定.结果表明,这9个微卫星位点均表现为高度或中度多态性；9个微卫星位点在选择的7组亲子中,获得了0.999 8～0.999 9的父权概率,说明这9个微卫星位点即使在亲缘关系较近的群体中进行亲子鉴定也是可行的.In the study, 9 STR loci（BM4621, BM143, OarHH55, OARJMP8, BM6438, BM6506, BM1824OarDB6, ILSTS004） of 132 paternal half-sib Chinese merino sheep were analyzed by microsatellite markers, and the distri-bution law and allele frequencies on those loci were calculated. Then paternity was identified for 7 parent-child groups ofthe 132 Chinese merino sheep. The results showed that the 9 microsatellite loci were characterized by high or moderate pol-ymorphism, and 9 microsatellite loci in the seven groups selected in paternity test revealed the patriarchal probabilities of 99. 98% -99.99%, suggesting that it is feasible to apply microsatellite marker in paternity identification of the groupswhich have close genetic relationships.

关 键 词：STR基因座 分子标记 绵羊 亲子鉴定 

分 类 号：S826.86[农业科学—畜牧学]