微囊藻毒素LR对大鼠卵巢颗粒细胞氧化损伤和凋亡的影响  

Effects of microcystins on the oxidative damage and apoptosis of ovarian granulosa cells in rats

作　　者：杨涛[1,2] 陈雨萍[1] 汪靖[1] 任南[1] 陈华[1] 

机构地区：[1]福建医科大学公共卫生学院,福建福州350004 [2]厦门市疾病预防控制中心,福建厦门361021

出　　处：《癌变．畸变．突变》2014年第1期16-19,23,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：福建省自然科学基金项目(2011J01180)

摘　　要：目的:研究微囊藻毒素LR(MC-LR)对大鼠卵巢颗粒细胞的氧化损伤和致凋亡作用。方法:分别以10、20、30μg/mL的MC-LR对体外培养的大鼠卵巢颗粒细胞染毒48 h,测定细胞内活性氧(ROS)含量、细胞培养液超氧化物歧化酶(T-SOD)活性、丙二醛(MDA)含量、卵巢颗粒细胞凋亡率和凋亡相关基因Bax、Bcl-2 mRNA的表达水平。结果:与对照组比较,随着MCLR剂量升高,染毒组细胞内ROS升高(r=0.925,<0.01);细胞培养液中T-SOD活性降低(=-0.865,P<0.05);MDA含量升高s s(r=0.811,P<0.01)。卵巢颗粒细胞凋亡率随MC-LR剂量的升高而升高(P<0.05);凋亡相关基因Bax mRNA的表达量随MC-LR剂量的s升高而升高(r=0.972,P<0.01),但Bcl-2 mRNA的表达量随MC-LR剂量的升高而降低(r=-0.972,P<0.01)。结论:MC-LR对大鼠卵s s巢颗粒细胞具有氧化损伤作用,并能引起卵巢颗粒细胞凋亡和凋亡相关基因Bax、Bcl-2 mRNA表达的改变。OBJECTIVE：To study the effects and mechanism of microcystin LR（MC-LR）on oxidative damage and apoptosis in rat ovarian granulosa cells. METHODS：After 48 h exposure to MC-LR at 0,10,20 and 30μg/mL,the level of intracelluar reactive oxygen species, superoxide dismutase （T-SOD） activity and MDA in culture medium,apoptosis and the mRNA changes of Bax and Bcl-2 expressions were evaluated. RESULTS：After MC-LR exposure at the dosage of 10,20 and 30μg/mL for 48 h,the T-SOD activity was significantly suppressed （P〈0.05）. The concentrations of ROS and MDA in the 20 μg/mL and 30μg/ml groups were significantly reduced （P〈0.05） when compared with control group. The correlation coefficient between dosage of MC-LR and ROS was 0.925（P〈0.01）,between dosage of MC-LR and MDA was 0.811（P〈0.01）,between dosage of MC-LR and T-SOD was-0.865（P〈0.05）. Cell assay indicated that the expression of Bax was up-regulated（P〈0.05） and Bcl-2 down- regulated （P〈0.05）. The correlation coefficient between dosage of MC-LR and Bax was 0.972（P〈0.01）,between dosage of MC-LR and Bcl-2 was -0.972 （P〈0.01）. CONCLUSION：MC-LR was capable of inducing oxidative damage of rat granulosa cells in vitro. MC-LR could cause apoptosis and changes in the expresons of Bax and Bcl-2.

关 键 词：微囊藻毒素LR 卵巢颗粒细胞 氧化损伤 细胞凋亡 BAX BCL-2 

分 类 号：R994.6[医药卫生—毒理学]